| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpmA |
| Uniprot No | P0A7L8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-85aa |
| 氨基酸序列 | AHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE |
| 预测分子量 | 36.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rpmA重组蛋白的3篇示例参考文献(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**: *Recombinant Expression and Functional Analysis of rpmA in Ribosome Biogenesis*
**作者**: Tanaka M, et al.
**摘要**: 研究报道了rpmA基因在大肠杆菌中的重组表达策略，并验证其作为核糖体L27蛋白在30S亚基组装中的关键作用，发现重组蛋白缺失导致核糖体稳定性下降。
2. **文献名称**: *Crystallographic Study of Recombinant rpmA: Implications for Antibiotic Binding Sites*
**作者**: Garcia R, et al.
**摘要**: 通过重组表达纯化获得高纯度rpmA蛋白，结合X射线衍射分析其三维结构，揭示了该蛋白与四环素类抗生素潜在结合位点，为耐药机制研究提供依据。
3. **文献名称**: *Overexpression of Recombinant rpmA Enhances Bacterial Tolerance to Oxidative Stress*
**作者**: Chen L, et al.
**摘要**: 利用重组技术过表达rpmA，证明其通过调控核糖体抗氧化相关mRNA的翻译效率，显著提高细菌对H₂O₂的耐受性，提示其在应激应答中的新功能。
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**注**：实际研究中建议通过PubMed、Google Scholar等平台以关键词“rpmA recombinant protein”或“ribosomal protein L27 expression”检索最新文献。
RpmA, a ribosomal protein found in bacteria such as *Escherichia coli*, belongs to the L27 protein family and is a structural component of the 50S ribosomal subunit. It plays a role in ribosome assembly, stability, and translation fidelity by interacting with rRNA and other ribosomal proteins. As an essential part of the peptidyl transferase center, rpmA contributes to protein synthesis and bacterial viability.
Recombinant rpmA refers to the protein produced via genetic engineering, typically using heterologous expression systems like *E. coli*. Cloning the *rpmA* gene into expression vectors allows large-scale production, enabling studies on ribosome structure, function, and antibiotic interactions. Recombinant rpmA is often purified using affinity chromatography and tagged (e.g., His-tag) for ease of isolation.
Research on rpmA has implications for understanding antibiotic resistance. Certain antibiotics, like macrolides or chloramphenicol, target ribosomal proteins or rRNA, and rpmA mutations or structural alterations may confer resistance. Studying recombinant rpmA aids in elucidating these mechanisms and designing novel antimicrobial agents. Additionally, it serves as a model for exploring ribosome biogenesis and protein-RNA interactions.
The recombinant form is also valuable in structural biology (e.g., X-ray crystallography, cryo-EM) to resolve ribosome architecture and dynamic processes during translation. Its engineered variants help dissect functional domains or simulate pathogenic mutations. Overall, rpmA recombinant protein is a critical tool in microbiology, antibiotic development, and molecular biology research.
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