| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOSC5 |
| Uniprot No | Q9NQT4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-235aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MMEEETHTDA KIRAENGTGS SPRGPGCSLR HFACEQNLLS RPDGSASFLQ GDTSVLAGVY GPAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV LQVVSDAGSL LACCLNAACM ALVDAGVPMR ALFCGVACAL DSDGTLVLDP TSKQEKEARA VLTFALDSVE RKLLMSSTKG LYSDTELQQC LAAAQAASQH VFRFYRESLQ RRYSKS |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXOSC5重组蛋白的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**: *Structural and functional analysis of the human exosome subunit hRrp46*
**作者**: Liu Q, et al.
**摘要**: 研究解析了人源外切体亚基hRrp46(EXOSC5)的重组蛋白结构，通过X射线晶体学揭示其与RNA结合的关键结构域，并证明其在RNA降解中的催化辅助作用。
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2. **文献名称**: *Recombinant expression and biochemical characterization of the EXOSC5 subunit of the human RNA exosome complex*
**作者**: Schmidt C, et al.
**摘要**: 报道了EXOSC5重组蛋白在大肠杆菌中的高效表达与纯化方法，通过体外酶活实验验证其参与外切体复合体对异常RNA底物的3'-5'方向降解功能。
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3. **文献名称**: *EXOSC5 mutations alter ribosome-associated quality control pathways in neurodegenerative disease*
**作者**: Hakobyan A, et al.
**摘要**: 利用重组EXOSC5蛋白模型，揭示其突变导致核糖体相关RNA质量控制失调，可能与神经退行性疾病中异常蛋白聚集的分子机制相关。
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注：以上文献信息为虚拟概括，实际研究中建议通过PubMed或Google Scholar以关键词“EXOSC5 recombinant”检索真实文献。
EXOSC5. also known as Rrp46 or p10. is a critical subunit of the evolutionarily conserved exosome complex, a multi-protein machinery responsible for RNA processing, degradation, and quality control in eukaryotic cells. As a core component of the exosome’s nine-subunit catalytically inactive ring structure, EXOSC5 plays a structural and regulatory role in maintaining the complex’s stability and guiding substrate specificity. It belongs to the S1/KH-domain protein family, characterized by RNA-binding motifs that facilitate interactions with RNA molecules during 3'→5' exoribonuclease activities mediated by associated catalytic subunits (e.g., DIS3/RRP44).
Recombinant EXOSC5 protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, enabling detailed biochemical and functional studies. Its recombinant form allows researchers to investigate exosome assembly, RNA-binding properties, and interactions with partner proteins or cofactors. Mutational analyses using recombinant EXOSC5 have helped identify residues critical for RNA recognition and complex integrity. Dysregulation of exosome components, including EXOSC5. has been linked to human diseases, such as pontocerebellar hypoplasia, neurodegenerative disorders, and cancers, making this protein a focus for therapeutic exploration. Recombinant EXOSC5 is also utilized in structural studies (e.g., cryo-EM) to map exosome architecture and in high-throughput screens for small-molecule inhibitors targeting exosome-related pathologies. Its production often includes affinity tags (e.g., His-tag) for purification, ensuring high yield and purity for experimental reproducibility. Research on EXOSC5 continues to shed light on RNA metabolism mechanisms and their implications in cellular homeostasis and disease.
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