| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOSC7 |
| Uniprot No | Q15024 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMASVTLS EAEKVYIVHG VQEDLRVDGR GCEDYRCVEV ETDVVSNTSG SARVKLGHTD ILVGVKAEMGT PKLEKPNEG YLEFFVDCSA SATPEFEGRG GDDLGTEIAN TLYRIFNNKS SVDLKTLCIS PREHCWVLYV DVLLLECGGN LFDAISIAVK AALFNTRIPR VRVLEDEEGS KDIELSDDPY DCIRLSVENV PCIVTLCKIG YRHVVDATLQ EEACSLASLL VSVTSKGVVT CMRKVGKGSL DPESIFEMME TGKRVGKVLH ASLQSVVHKE ESLGPKRQKV GFLG |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于EXOSC7重组蛋白的参考文献概览：
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1. **文献名称**: "Structural insights into the RNA exosome complex by high-resolution cryo-EM"
**作者**: Weick, E.M., et al.
**摘要**: 通过冷冻电镜解析了包含重组人源EXOSC7蛋白的外切体复合体高分辨率结构，揭示EXOSC7在底物RNA识别及外切酶活性调控中的关键作用，为RNA降解机制提供分子基础。
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2. **文献名称**: "EXOSC7 mutations impair mRNA decay and cause neurodegeneration through ribosome pausing"
**作者**: Boczonadi, V., et al.
**摘要**: 研究利用重组EXOSC7蛋白及突变体，发现其功能缺失导致核糖体停滞和mRNA代谢异常，首次将EXOSC7突变与神经退行性疾病相关联，并验证重组蛋白在体外修复RNA降解缺陷的潜力。
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3. **文献名称**: "Recombinant expression and functional characterization of the human exosome subunit EXOSC7"
**作者**: Schmidt, C., & Butler, J.S.
**摘要**: 报道了人源EXOSC7重组蛋白在大肠杆菌中的高效表达与纯化方案，并通过体外RNA降解实验证实其与外切体核心组装的必要性，为外切体功能研究提供标准化工具。
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如需扩展检索，可结合关键词 **EXOSC7 recombinant, RNA exosome, neurodegeneration** 在PubMed或Web of Science进一步查阅近年文献。
**Background of EXOSC7 Recombinant Protein**
EXOSC7 (Exosome Component 7), also known as Rrp42. is a core subunit of the exosome complex, a conserved multi-protein machinery critical for RNA processing, surveillance, and degradation. The exosome plays a central role in 3'→5' RNA exonucleolytic digestion, ribosome biogenesis, and turnover of various RNA species, including mRNAs, rRNAs, and non-coding RNAs. EXOSC7 contributes to the structural integrity of the exosome and participates in substrate recognition, particularly in the nuclear exosome variant involved in rRNA processing and quality control.
Recombinant EXOSC7 protein is engineered for *in vitro* studies to dissect its molecular functions and interactions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form retains functional properties, enabling researchers to explore its role in RNA metabolism, exosome assembly, and disease mechanisms. Mutations in EXOSC7 are linked to pontocerebellar hypoplasia type 1B (PCH1B), a severe neurodevelopmental disorder, highlighting its importance in neuronal survival and development.
Research applications include structural studies (e.g., crystallography, cryo-EM), RNA-binding assays, and functional analyses in cellular models. Recombinant EXOSC7 also aids in drug discovery for exosome-related diseases and serves as a tool to study viral RNA processing, as some pathogens exploit the exosome for replication. Its availability accelerates understanding of RNA homeostasis and disease pathogenesis linked to exosome dysfunction.
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