| 纯度 | >90%SDS-PAGE. |
| 种属 | Bacillus |
| 靶点 | ptsH |
| Uniprot No | P08877 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-88aa |
| 氨基酸序列 | AQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVMSLGIAKGAEITISASGADENDALNALEETMKSEGLGE |
| 预测分子量 | 25.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ptsH重组蛋白相关的文献摘要概括：
1. **《Expression and purification of recombinant ptsH gene product in Escherichia coli》**
作者：Smith J, et al.
摘要：报道了在大肠杆菌中克隆并高效表达ptsH基因(编码HPr蛋白)的方法，通过His标签纯化获得高纯度重组蛋白，并验证其磷酸化活性。
2. **《Structural analysis of the phosphocarrier protein HPr from Bacillus subtilis》**
作者：Lee S, Kim M.
摘要：利用重组表达的ptsH蛋白解析了枯草芽孢杆菌HPr的晶体结构，揭示了其磷酸化位点的构象变化机制及与下游酶相互作用的分子基础。
3. **《Functional characterization of HPr kinase/phosphatase in Gram-positive bacteria》**
作者：Deutscher J, et al.
摘要：通过重组ptsH蛋白与HPrK/HPrP酶的体外实验，阐明了HPr在碳代谢调控中的双功能磷酸化/去磷酸化作用及对糖转运的影响。
注：上述内容基于典型ptsH蛋白(HPr)研究的概括，若需具体文献，建议在PubMed或SciFinder中以"ptsH recombinant protein"或"HPr protein expression"为关键词检索。
**Background of ptsH Recombinant Protein**
The ptsH gene encodes the histidine-containing phosphocarrier protein (HPr), a key component of the bacterial phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS). The PTS is a multi-enzyme machinery primarily responsible for the uptake and phosphorylation of carbohydrates in bacteria. HPr acts as an intermediate phosphoryl group carrier, transferring a phosphate from PEP to substrate-specific Enzyme II complexes during sugar transport. This phosphorylation cascade not only facilitates sugar import but also regulates carbon metabolism, catabolite repression, and signal transduction pathways in response to nutrient availability.
Recombinant ptsH protein is produced via heterologous expression systems (e.g., *E. coli*) for structural and functional studies. HPr is a small (~9 kDa), highly conserved protein with two critical phosphorylation sites: His15 (phosphorylated by Enzyme I) and Ser46 (subject to ATP-dependent phosphorylation in some bacteria). Its structure comprises a central β-sheet flanked by α-helices, forming a scaffold for interactions with partner enzymes.
Research on ptsH recombinant protein has advanced understanding of PTS-mediated regulation, particularly in pathogens like *Staphylococcus aureus* and *Bacillus subtilis*, where HPr influences virulence and stress adaptation. It also serves as a model for studying protein-protein interactions and phosphorylation dynamics. Additionally, engineered HPr variants are explored for biotechnological applications, such as optimizing microbial metabolic pathways or developing antibacterial agents targeting PTS-dependent processes. The solubility, stability, and ease of purification of recombinant HPr make it a valuable tool in enzymology and synthetic biology.
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