纯度 | >90%SDS-PAGE. |
种属 | E.coli |
靶点 | rpsB |
Uniprot No | P0A7V0 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 2-241aa |
氨基酸序列 | ATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDALFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEAE |
预测分子量 | 53.6 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与rpsB重组蛋白相关的文献摘要信息(注:文献为模拟示例,实际引用需核实原文):
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1. **"Expression and Purification of Recombinant Ribosomal Protein S2 (rpsB) in Escherichia coli"**
*作者:Smith J et al. (2015)*
摘要:研究报道了通过构建pET载体在大肠杆菌中高效表达重组rpsB蛋白,优化了诱导条件与镍柱纯化步骤,获得高纯度蛋白用于后续结构分析。
2. **"Structural Insights into rpsB Function in Bacterial Translation Initiation"**
*作者:Li X et al. (2018)*
摘要:利用重组rpsB蛋白进行X射线晶体学研究,解析其三维结构,揭示了rpsB在核糖体组装及mRNA识别中的关键作用域。
3. **"rpsB Interactions with Antibiotic Targets: A Biochemical Study"**
*作者:Garcia R et al. (2020)*
摘要:通过体外结合实验发现,重组rpsB蛋白与特定氨基糖苷类抗生素直接互作,为细菌耐药性机制提供了分子层面的解释。
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建议通过PubMed或Google Scholar以关键词 **"rpsB recombinant protein"** 或 **"ribosomal protein S2 expression"** 检索最新文献获取准确信息。
**Background of RpsB Recombinant Protein**
RpsB, a ribosomal protein encoded by the *rpsB* gene, is a critical component of the 30S subunit in prokaryotic ribosomes. As part of the small ribosomal subunit, RpsB (also known as ribosomal protein S2) plays a pivotal role in translation initiation and mRNA binding during protein synthesis. It contributes to ribosome assembly, structural stability, and interactions with other translational machinery components. In *Escherichia coli*, RpsB is essential for cell viability, underscoring its fundamental role in cellular function.
The recombinant RpsB protein is produced through genetic engineering, typically by cloning the *rpsB* gene into expression vectors (e.g., plasmid systems) and expressing it in heterologous hosts like *E. coli*. This approach allows large-scale production of the protein with high purity for functional and structural studies. Recombinant RpsB retains its native folding and biochemical properties, enabling researchers to investigate its interactions with rRNA, mRNA, or antibiotics, as well as its role in ribosome biogenesis.
Interest in RpsB extends to its potential as a drug target. Studies have explored its involvement in bacterial stress responses and antibiotic resistance mechanisms. For example, mutations in *rpsB* can affect bacterial susceptibility to aminoglycosides or tetracyclines. Additionally, recombinant RpsB serves as a tool for structural biology (e.g., X-ray crystallography or cryo-EM) to elucidate ribosome architecture and dynamics. Beyond prokaryotes, homologs of RpsB exist in eukaryotic organelles like mitochondria and chloroplasts, highlighting its evolutionary conservation.
Overall, RpsB recombinant protein is a valuable resource for advancing our understanding of translation mechanisms, antimicrobial development, and ribosomal function across life forms.
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