| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FABP1 |
| Uniprot No | P07148 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-127aa |
| 氨基酸序列 | MSFSGKYQLQSQENFEAFMKAIGLPEELIQKGKDIKGVSEIVQNGKHFKFTITAGSKVIQNEFTVGEECELETMTGEKVKTVVQLEGDNKLVTTFKNIKSVTELNGDIITNTMTLGDIVFKRISKRI |
| 预测分子量 | 16.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FABP1重组蛋白的3篇代表性文献示例(注：文献为虚构示例，仅作格式参考)：
1. **文献名称**：*Expression and Purification of Recombinant Human FABP1 in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：报道了通过大肠杆菌系统高效表达可溶性FABP1重组蛋白的优化方法，纯化后蛋白具有与天然蛋白相似的脂肪酸结合活性。
2. **文献名称**：*Structural Insights into FABP1 Ligand Binding Using Recombinant Protein Crystallography*
**作者**：Zhang L, et al.
**摘要**：利用重组FABP1解析其晶体结构，揭示了与长链脂肪酸结合的关键氨基酸位点及构象变化机制。
3. **文献名称**：*Functional Analysis of Recombinant FABP1 in Lipid Metabolism Disorders*
**作者**：Tanaka K, et al.
**摘要**：通过体外实验验证重组FABP1对脂质转运的调控作用，并探讨其在代谢性疾病模型中的潜在治疗价值。
(提示：实际文献需通过PubMed或Google Scholar检索关键词如“recombinant FABP1”、“FABP1 expression”等获取。)
Fatty Acid-Binding Protein 1 (FABP1), also known as liver-type FABP (L-FABP), is a small cytoplasmic protein predominantly expressed in the liver and intestinal epithelium. It belongs to the fatty acid-binding protein (FABP) family, which plays a critical role in intracellular lipid transport, metabolism, and signaling. FABP1 binds hydrophobic ligands such as long-chain fatty acids, bile acids, and phospholipids, facilitating their solubilization and trafficking within cells. Its structure features a β-barrel fold with a central ligand-binding pocket, enabling reversible interactions with diverse lipid molecules.
Recombinant FABP1 is engineered using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to produce purified, functional protein for research and therapeutic applications. Its recombinant form retains native ligand-binding properties, making it a valuable tool for studying lipid metabolism, drug interactions, and metabolic disorders. Researchers utilize it to investigate mechanisms underlying non-alcoholic fatty liver disease (NAFLD), atherosclerosis, and obesity, as dysregulated FABP1 expression correlates with lipid accumulation and inflammation.
In drug development, recombinant FABP1 serves as a target or biomarker for metabolic syndrome and inflammatory bowel diseases. It also aids in structural studies to design small-molecule inhibitors or modulators. Additionally, its role in cellular detoxification and oxidative stress response has sparked interest in cancer and neurodegenerative disease research. The production of recombinant FABP1 ensures consistency, scalability, and customization (e.g., tagging for detection), addressing limitations of tissue-derived protein isolation. As a multifunctional mediator of lipid signaling, recombinant FABP1 remains pivotal in both basic science and translational studies targeting lipid-related pathologies.
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