| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBMY1F |
| Uniprot No | Q15415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MVEADHPGKLFIGGLNRETNEKMLKAVFGKHGPISEVLLIKDRTSKSRGFAFITFENPADAKNAAKDMNGTSLHGKAIKVEQAKKPSFQSGGRRRPPASSRNRSPSGSLRSARGSSGGTRGWLPSHEGHLDDGGYTPDLKMSYSRGLIPVKRGPSSRSGGPPPKKSAPSAVARSNSWMGSQGPMSQRRENYGVPPRRATISSWRNDRMSTRHDGYATNDGNHPSCQETRDYAPPSRGYAYRDNGHSNRDEHSSRGYRNHRSSRETRDYAPPSRGHAYRDYGHSRRDESYSRGYRNHRSSRETREYAPPSRGHGYRDYGHSRRHESYSRGYRNHPSSRETRDYAPPHRDYAYRDYGHSSWDEHSSRGYSYHDGYGEALGRDHSEHLSGSSYRDALQRYGTSHGAPPARGPRMSYGGSTCHAYSNTRDRYGRSWESYSSCGDFHYCDREHVCRKDQRNPPSLGRVLPDPREAYGSSSYVASIVDGGESRSEKGDSSRY |
| 预测分子量 | 71.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBMY1F重组蛋白的示例参考文献(部分为模拟概括，实际文献需通过学术数据库检索)：
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1. **文献名称**: *RBMY1F, a novel germ cell-specific RNA-binding protein, promotes spermatogenesis through alternative splicing regulation*
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌系统中重组表达并纯化了RBMY1F蛋白，发现其通过结合特定RNA序列调控精子发生相关基因的剪接。功能实验表明，RBMY1F缺失导致小鼠精子形成异常。
2. **文献名称**: *Functional characterization of recombinant RBMY1F in male fertility*
**作者**: Singh K, et al.
**摘要**: 作者利用哺乳动物细胞系重组表达RBMY1F，证实其与DAZ家族蛋白相互作用，并参与减数分裂中RNA稳定性调控。研究支持RBMY1F在人类男性不育中的潜在作用。
3. **文献名称**: *Structural insights into the RNA-binding domain of RBMY1F*
**作者**: Tanaka H, et al.
**摘要**: 通过X射线晶体学解析了重组RBMY1F蛋白的RNA结合域结构，揭示了其特异性识别靶标RNA的分子机制，为理解Y染色体编码蛋白的生殖功能提供依据。
4. **文献名称**: *RBMY1F as a cancer/testis antigen: Expression and immunogenicity in human tumors*
**作者**: Wang Y, et al.
**摘要**: 研究在多种癌症组织中检测到RBMY1F重组蛋白的异常表达，并证明其可诱导特异性T细胞反应，提示其作为癌症免疫治疗靶点的潜力。
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**注**：以上为模拟示例，实际文献建议通过PubMed、Web of Science等平台检索关键词“RBMY1F recombinant protein”“RBMY1F function”或“Y chromosome RNA-binding proteins”。真实研究多集中于男性生殖发育及癌症睾丸抗原方向。
RBMY1F, a member of the RNA-binding motif (RBM) protein family, is a Y chromosome-encoded protein predominantly expressed in male germ cells. This protein belongs to the RBMY subfamily, which shares homology with the autosomal RBMX protein but has evolved testis-specific functions through gene duplication and divergence. Structurally, RBMY1F contains an N-terminal RNA recognition motif (RRM) and a C-terminal SR-rich domain, features characteristic of proteins involved in RNA processing and splicing regulation.
Functionally, RBMY1F is implicated in spermatogenesis, particularly during meiotic and post-meiotic stages of germ cell development. It interacts with spliceosomal components and participates in alternative splicing events crucial for male germ cell maturation. Studies suggest its involvement in maintaining the stability and translation of mRNAs required for sperm formation, potentially through phase separation mechanisms mediated by its intrinsically disordered regions.
The recombinant RBMY1F protein, typically produced in bacterial or mammalian expression systems, serves as a vital tool for investigating male fertility mechanisms. Researchers utilize it to study protein-RNA interactions, map functional domains, and develop antibodies for diagnostic applications. Its abnormal expression has been associated with spermatogenic failure and testicular pathologies, making it a potential biomarker for male infertility. Recent investigations also explore its ectopic expression in certain cancers, particularly male-specific malignancies, though its oncogenic role remains under debate. Current research focuses on elucidating its post-translational modifications and tissue-specific interaction networks, which may reveal novel therapeutic targets for reproductive disorders.
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