| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | omp25 |
| Uniprot No | P57105 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-145aa |
| 氨基酸序列 | MNGRVDYLVTEEEINLTRGPSGLGFNIVGGTDQQYVSNDSGIYVSRIKENGAAALDGRLQ EGDKILSVNGQDLKNLLHQDAVDLFRNAGYAVSLRVQHRLQVQNGPIGHRGEGDPSGIPI FMVLVPVFALTMVAAWAFMRYRQQL |
| 预测分子量 | 15,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Omp25重组蛋白的3篇代表性文献的简要信息(注：内容基于模拟数据，实际文献需通过学术数据库查询)：
1. **文献名称**：*Expression and Immunogenicity of Recombinant Omp25 from Brucella spp.*
**作者**：Smith A, et al.
**摘要**：研究成功在大肠杆菌中表达重组Omp25蛋白，并证明其在小鼠模型中可诱导强烈的Th1型免疫反应，提示其作为布鲁氏菌亚单位疫苗的潜力。
2. **文献名称**：*Omp25 Recombinant Protein as a Diagnostic Antigen for Brucellosis*
**作者**：Zhang L, et al.
**摘要**：评估重组Omp25作为诊断抗原的性能，结果显示其与布鲁氏菌感染者血清的高特异性结合，可用于开发血清学检测方法。
3. **文献名称**：*Structural and Functional Characterization of Brucella Omp25 in Host-Pathogen Interaction*
**作者**：García E, et al.
**摘要**：通过体外实验证实重组Omp25蛋白与宿主细胞受体的相互作用，揭示其在布鲁氏菌入侵过程中的关键作用，为抗感染策略提供依据。
如需具体文献，建议通过PubMed或Web of Science检索关键词“Omp25 recombinant protein”获取最新研究。
Omp25 recombinant protein is a genetically engineered version of the outer membrane protein 25 (Omp25) found in certain Gram-negative bacteria, particularly in *Brucella* species, which are zoonotic pathogens causing brucellosis. Native Omp25 is a 25 kDa immunogenic protein localized in the bacterial outer membrane, playing roles in virulence, host-pathogen interactions, and immune evasion. Its recombinant form is produced using heterologous expression systems (e.g., *E. coli*) by cloning the *omp25* gene into expression vectors, followed by purification via chromatography techniques.
Research on recombinant Omp25 focuses on its potential applications in diagnostics, vaccine development, and immunological studies. It serves as a target antigen in serological assays (e.g., ELISA) to detect *Brucella*-specific antibodies, aiding in disease surveillance. As a vaccine candidate, Omp25 has been explored for its ability to induce protective immune responses, though its efficacy varies depending on the bacterial strain and delivery platform. Studies also investigate its role in modulating host immune responses, including interactions with Toll-like receptors (TLRs) and cytokine regulation.
Despite its promise, challenges remain, such as antigenic variability across *Brucella* species and the need for adjuvant optimization. Recent advances in structural biology and bioinformatics have improved understanding of its epitope mapping and stability, guiding rational vaccine design. Overall, Omp25 recombinant protein represents a valuable tool for combating brucellosis and studying bacterial pathogenesis.
×
