| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | C11orf73 |
| Uniprot No | Q53FT3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPDSNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDSMAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKWYENFQRRLAQNPLFWKT |
| 预测分子量 | 37.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C11orf73重组蛋白的模拟参考文献示例(建议通过PubMed或专业数据库核实真实文献)：
1. **文献名称**: *C11orf73 regulates ciliogenesis and mitochondrial dynamics in human cells*
**作者**: Smith A, et al.
**摘要**: 研究揭示了C11orf73重组蛋白通过调控微管稳定性参与纤毛形成，并影响线粒体网络完整性，暗示其在发育障碍中的潜在作用。
2. **文献名称**: *Functional characterization of C11orf73 as a novel DNA damage response protein*
**作者**: Chen L, et al.
**摘要**: 通过重组蛋白表达实验，发现C11orf73与BRCA1复合物相互作用，在DNA双链断裂修复中起辅助作用，可能影响癌症治疗敏感性。
3. **文献名称**: *C11orf73 interacts with the intraflagellar transport system and is essential for spermatogenesis*
**作者**: Wang Y, et al.
**摘要**: 在小鼠模型中，C11orf73重组蛋白被证实与鞭毛内运输蛋白IFT20结合，其缺失导致精子发育缺陷和不育表型。
4. **文献名称**: *C11orf73 overexpression promotes hepatocellular carcinoma progression via mTOR signaling*
**作者**: Tanaka K, et al.
**摘要**: 研究显示C11orf73重组蛋白在肝癌中异常高表达，并通过激活mTOR通路促进肿瘤细胞增殖和侵袭。
**注意**：以上文献为模拟示例，实际文献需通过学术数据库检索。建议结合关键词“C11orf73 recombinant protein”或“C11orf73 function”在PubMed、Web of Science等平台查询最新研究。
The C11orf73 gene, located on chromosome 11 open reading frame 73. encodes a protein with emerging but incompletely characterized roles in cellular processes. Though its precise molecular functions remain under investigation, C11orf73 has been implicated in mitotic regulation and chromosomal stability. Studies suggest potential interactions with centromere-associated proteins, hinting at a role in spindle assembly or chromosome segregation during cell division. Its dysregulation has been loosely associated with certain cancers and developmental disorders, though mechanistic links require further validation.
Recombinant C11orf73 protein is engineered for in vitro studies, typically expressed in bacterial, insect, or mammalian systems with affinity tags (e.g., His-tag, GST) to facilitate purification. This synthetic approach enables controlled investigation of its biochemical properties, post-translational modifications, and interaction networks. Structural analyses predict coiled-coil domains, which may mediate protein-protein interactions critical to its function. Researchers utilize recombinant C11orf73 to develop antibodies, map binding partners via pull-down assays, and study its localization dynamics in cellular models.
Current research gaps include clarifying its role in centromere biology and potential connections to mitotic errors. The recombinant protein serves as a crucial tool for functional studies, particularly in dissecting its contribution to genome integrity and potential therapeutic relevance in proliferation-related diseases. Standardized production of bioactive C11orf73 recombinant protein remains challenging due to its inherent physicochemical properties, prompting optimization of expression and purification protocols.
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