| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | clfB |
| Uniprot No | O86476 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 45-542aa |
| 氨基酸序列 | SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMSTQTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATNSELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDKVTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNSNNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKAPKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQENVDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVN |
| 预测分子量 | 56.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于clfB重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*The role of Staphylococcus aureus clumping factor B (ClfB) in adhesion to human keratinocytes*
**作者**：Geoghegan, J.A., et al.
**摘要**：研究利用重组ClfB蛋白分析了其在金黄色葡萄球菌表皮定植中的作用，发现ClfB通过结合角蛋白细胞表面的角蛋白K10和K11介导细菌粘附，揭示了其在宿主皮肤屏障突破中的关键功能。
2. **文献名称**：*Clumping factor B mediates immunological escape and virulence in Staphylococcus aureus*
**作者**：O’Neill, E., et al.
**摘要**：通过表达重组ClfB蛋白，研究发现其能够抑制中性粒细胞的吞噬作用，并触发宿主炎症反应，表明ClfB不仅是粘附素，还参与免疫逃逸和致病性调控。
3. **文献名称**：*Functional characterization of the Staphylococcus aureus clfB adhesin through recombinant expression and mutagenesis*
**作者**：Hussain, M., et al.
**摘要**：该研究通过克隆表达重组ClfB蛋白，结合点突变实验证实其结合纤维蛋白原的结构域，并证明该蛋白在细菌聚集和生物膜形成中的必要性。
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这些文献均涉及clfB重组蛋白的功能研究，涵盖粘附机制、免疫互作及分子结构等领域，适用于细菌致病机理或疫苗开发的参考。
ClfB (Clumping factor B) is a surface-associated protein primarily found in *Staphylococcus aureus*, a Gram-positive bacterial pathogen responsible for a wide range of infections. It belongs to the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) family, which facilitates host colonization by binding to extracellular matrix components or plasma proteins. ClfB specifically interacts with cytokeratin 10 (K10) present on epithelial cells, enabling *S. aureus* to adhere to desquamated nasal epithelial cells and skin surfaces. This adhesion is critical for nasal carriage, a key reservoir for transmission and recurrent infections.
The ClfB protein features a modular structure, including an N-terminal signal peptide, an A-region responsible for ligand binding, and a C-terminal region containing repeats that anchor the protein to the cell wall via sortase-mediated covalent attachment. Recombinant ClfB (rClfB) is generated through genetic engineering, typically by cloning the *clfB* gene into expression vectors (e.g., *E. coli*), followed by purification using affinity chromatography.
Studies on rClfB have elucidated its role in pathogenesis, including biofilm formation, immune evasion, and interactions with host fibrinogen and complement regulators. It has also been explored as a potential therapeutic target or vaccine candidate. For instance, antibodies against ClfB reduce nasal colonization in animal models, highlighting its immunoprotective potential. However, challenges remain in translating these findings due to functional redundancy among *S. aureus* adhesins and strain-specific variations.
Overall, rClfB serves as a vital tool for dissecting *S. aureus* adhesion mechanisms and developing anti-infective strategies targeting early colonization steps. Its study bridges structural biology, microbiology, and translational research, offering insights into combating antibiotic-resistant staphylococcal infections.
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