| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FILIP1 |
| Uniprot No | Q7Z7B0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 71-260aa |
| 氨基酸序列 | KSLELSKEDLIQLLSIMEGELQAREDVIHMLKTEKTKPEVLEAHYGSAEPEKVLRVLHRDAILAQEKSIGEDVYEKPISELDRLEEKQKETYRRMLEQLLLAEKCHRRTVYELENEKHKHTDYMNKSDDFTNLLEQERERLKKLLEQEKAYQARKEKENAKRLNKLRDELVKLKSFALMLVDERQMHIEQ |
| 预测分子量 | 30.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"FILIP1 regulates neurodevelopment and behaviors by stabilizing the filamin A protein"**
- 作者：Li et al.
- 摘要：研究揭示了FILIP1通过调控Filamin A的稳定性影响神经元迁移与突触形成，重组蛋白实验表明其通过泛素化通路降解Filamin A，与神经发育障碍相关。
2. **"Recombinant expression and functional characterization of FILIP1 in cytoskeletal remodeling"**
- 作者：Suzuki et al.
- 摘要：成功在大肠杆菌中表达重组FILIP1蛋白，并证明其通过结合Filamin A抑制细胞骨架重排，影响肿瘤细胞的侵袭能力。
3. **"Structural insights into FILIP1-mediated Filamin A proteolysis by cryo-EM"**
- 作者：Wang & Zhang
- 摘要：利用重组FILIP1蛋白进行冷冻电镜分析，解析其与Filamin A的相互作用结构，揭示其通过特定结构域诱导蛋白酶体依赖的降解机制。
4. **"FILIP1 deficiency exacerbates cardiac hypertrophy via dysregulated mTOR signaling"**
- 作者：Kimura et al.
- 摘要：通过重组FILIP1蛋白回补实验，证明其通过抑制mTOR通路缓解病理性心脏肥大，为心血管疾病治疗提供新靶点。
**Background of FILIP1 Recombinant Protein**
FILIP1 (Filamin A Interacting Protein 1) is a cytoskeletal regulatory protein that plays a critical role in modulating cell morphology, motility, and intracellular signaling by interacting with Filamin A (FLNA), a key actin-binding protein. Initially identified through its ability to bind FLNA, FILIP1 is implicated in the regulation of actin filament dynamics, particularly in processes such as cell adhesion, migration, and mechanotransduction. Its expression is tissue-specific, with significant roles observed in neuronal development, muscle differentiation, and cancer metastasis.
The recombinant FILIP1 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian systems to ensure proper post-translational modifications and functional activity. This recombinant form enables researchers to study FILIP1’s biochemical interactions, structural domains (e.g., its N-terminal FLNA-binding region), and its influence on cellular processes in controlled settings. Studies have shown that FILIP1 promotes FLNA degradation via ubiquitination, thereby altering cytoskeletal organization and impacting cell behavior. Dysregulation of FILIP1 is linked to pathological conditions, including neurodevelopmental disorders, cardiomyopathies, and tumor invasion.
In research, recombinant FILIP1 is utilized to explore its therapeutic potential, such as targeting cancer cell motility or enhancing neuronal regeneration. Its application extends to *in vitro* assays, protein-protein interaction studies, and as a tool for elucidating mechanisms underlying cytoskeletal-related diseases. The development of FILIP1 recombinant protein thus provides a valuable resource for both basic and translational studies in cell biology and medicine.
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