| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRR11 |
| Uniprot No | Q96HE9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MPKFKQRRRKLKAKAERLFKKKEASHFQSKLITPPPPPPSPERVGISSIDISQSRSWLTSSWNFNFPNIRDAIKLWTNRVWSIYSWCQNCITQSLEVLKDTIFPSRICHRELYSVKQQFCILESKLCKLQEALKTISESSSCPSCGQTCHMSGKLTNVPACVLITPGDSKAVLPPTLPQPASHFPPPPPPPPLPPPPPPLAPVLLRKPSLAKALQAGPLKKDGPMQITVKDLLTVKLKKTQSLDEKRKLIPSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLRKVDVERSPGGTPLTNKENMETGTGLTPVMTQALRRKFQLAHPRSPTPTLPLSTSSFDEQN |
| 预测分子量 | 56.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRR11重组蛋白的参考文献示例(注：以下内容为模拟示例，非真实文献，建议通过PubMed、Google Scholar等平台检索真实研究)：
---
1. **文献名称**: *PRR11重组蛋白在非小细胞肺癌中的功能及机制研究*
**作者**: 张伟等
**摘要**: 本研究通过构建PRR11重组蛋白，探究其在非小细胞肺癌细胞中的功能。实验表明，PRR11过表达可促进癌细胞增殖并调控G1/S期转换，其机制可能与结合Cyclin D1并激活CDK4通路相关。
2. **文献名称**: *PRR11重组蛋白与E2F信号通路的相互作用分析*
**作者**: Li, X. et al.
**摘要**: 作者利用重组PRR11蛋白进行体外结合实验，发现PRR11直接与E2F转录因子家族成员相互作用，增强靶基因启动子活性，提示PRR11在细胞周期转录调控中的关键作用。
3. **文献名称**: *PRR11重组蛋白的晶体结构解析及其在肝癌中的临床应用潜力*
**作者**: Chen, J. & Wang, Y.
**摘要**: 本文首次报道了PRR11重组蛋白的晶体结构，揭示了其C端结构域与肿瘤相关蛋白的结合位点。体外实验显示，抑制PRR11重组蛋白活性可显著抑制肝癌细胞迁移。
4. **文献名称**: *PRR11重组蛋白通过PI3K/AKT通路调控胃癌细胞凋亡*
**作者**: 李明等
**摘要**: 通过表达PRR11重组蛋白并沉默内源基因，研究发现PRR11通过激活PI3K/AKT信号通路抑制胃癌细胞凋亡，提示其作为潜在治疗靶点的可能性。
---
**提示**：实际文献需通过学术数据库(如NCBI PubMed、Web of Science)以关键词“PRR11 recombinant protein”“PRR11 oncogene”等检索获取。
PRR11 (Proline Rich 11) is a protein-coding gene first identified in studies exploring molecular mechanisms underlying cancer progression. It is located on chromosome 17q22 in humans and has garnered attention due to its potential role in cell cycle regulation, apoptosis, and tumorigenesis. PRR11 encodes a nuclear protein characterized by a proline-rich domain, which is often associated with protein-protein interactions and signaling pathway modulation. Research suggests PRR11 interacts with SKA2 (Spindle and Kinetochore Associated Complex Subunit 2), forming a functional complex critical for mitotic progression and chromosomal stability.
Elevated PRR11 expression has been observed in multiple cancers, including lung, breast, and hepatocellular carcinomas, correlating with poor prognosis. It is hypothesized to promote tumor cell proliferation, metastasis, and chemoresistance by dysregulating cell cycle checkpoints (e.g., G1/S transition) and activating oncogenic pathways like PI3K/AKT and Wnt/β-catenin. These findings position PRR11 as a potential diagnostic biomarker or therapeutic target.
Recombinant PRR11 protein, produced via bacterial or mammalian expression systems, enables functional studies to dissect its molecular interactions and mechanisms. It is widely used in in vitro assays, antibody development, and structural analyses. Recent studies also explore its role in non-cancer contexts, such as embryonic development and immune regulation, though these areas remain less characterized. Despite progress, the full scope of PRR11's biological functions and clinical relevance requires further investigation.
×
