| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM14 |
| Uniprot No | Q96PK6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-669aa |
| 氨基酸序列 | MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGALRAIEALHGHELRPGRALVVEMSRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGKRINVELSTKGQKKGPGLAVQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFFGRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMTAQAASYRAQPSVSLGAPYRGQLASPSSQSAAASSLGPYGGAQPSASALSSYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGAQAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYNAQPSASYNAQSAPYAAQQAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASMGLSGSYGAQSAAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGNAYDGAGQPSAAYLSMSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTKSSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQRRM |
| 预测分子量 | 85.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBM14重组蛋白的3篇代表性文献及其核心内容概括：
1. **文献名称**：**"RBM14 is essential for nuclear speckle formation and promotes oncogene expression by stabilizing mRNA"**
**作者**：Li, Y., et al.
**摘要**：该研究揭示了RBM14通过介导核斑(nuclear speckle)的形成调控基因表达的功能。研究发现，RBM14重组蛋白通过结合RNA和蛋白质互作维持核斑结构，并促进致癌基因(如c-Myc)的mRNA稳定性，从而驱动肿瘤发生。
2. **文献名称**：**"RBM14 interacts with the RNA exosome to regulate DNA damage response in cancer cells"**
**作者**：Wang, X., et al.
**摘要**：文章发现RBM14重组蛋白通过与RNA外泌体复合体相互作用，参与DNA损伤修复过程。实验表明，RBM14缺失会导致DNA损伤应答异常，并通过调控非编码RNA加工影响肿瘤细胞的放疗敏感性。
3. **文献名称**：**"Structural insights into RBM14-mediated transcriptional regulation in the Wnt/β-catenin pathway"**
**作者**：Chen, L., et al.
**摘要**：本研究解析了RBM14重组蛋白的RNA结合结构域(RBD)晶体结构，并揭示其通过结合Wnt信号通路相关mRNA(如β-catenin)增强转录活性的机制，为靶向RBM14的癌症治疗提供了结构基础。
(注：以上文献标题及内容为示例性概括，实际研究需参考具体论文数据。)
**Background of RBM14 Recombinant Protein**
RBM14 (RNA Binding Motif Protein 14), also known as CoAA or SYT1-interacting protein, is a multifunctional RNA-binding protein involved in transcriptional regulation, RNA processing, and DNA damage response. It belongs to the RRM (RNA recognition motif) family, characterized by its ability to interact with RNA and other proteins through distinct domains. Structurally, RBM14 contains two N-terminal RRM domains critical for RNA/DNA binding and a C-terminal prion-like domain implicated in protein-protein interactions and phase separation.
RBM14 plays a role in co-activating transcription by interacting with nuclear receptors and mediating chromatin remodeling. It is also involved in alternative splicing, mRNA stability, and the formation of stress granules under cellular stress. Studies highlight its dual role in cancer: as an oncogene, it promotes tumor growth and metastasis in cancers like hepatocellular carcinoma and breast cancer, while its loss or dysregulation is linked to genomic instability and impaired DNA repair.
The recombinant RBM14 protein is typically produced in *E. coli* or mammalian expression systems, ensuring proper post-translational modifications for functional studies. Purification methods often employ affinity tags (e.g., His-tag) for high yield and purity. Recombinant RBM14 is widely used in *in vitro* assays to study RNA-protein interactions, spliceosome assembly, and transcriptional co-regulation. It also serves as a tool for identifying binding partners or screening therapeutic agents targeting its oncogenic or phase-separation activities.
Dysregulation of RBM14 is associated with neurodegenerative diseases and cancer, making it a potential biomarker or therapeutic target. Ongoing research aims to elucidate its role in liquid-liquid phase separation and its impact on cellular stress responses, offering insights into novel treatment strategies.
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