首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGF14 |
| Uniprot No | Q92915 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-252aa |
| 氨基酸序列 | MVKPVPLFRRTDFKLLLCNHKDLFFLRVSKLLDCFSPKSMWFLWNIFSKGTHMLQCLCGKSLKKNKNPTDPQLKGIVTRLYCRQGYYLQMHPDGALDGTKDDSTNSTLFNLIPVGLRVVAIQGVKTGLYIAMNGEGYLYPSELFTPECKFKESVFENYYVIYSSMLYRQQESGRAWFLGLNKEGQAMKGNRVKKTKPAAHFLPKPLEVAMYREPSLHDVGETVPKPGVTPSKSTSASAIMNGGKPVNKSKTT |
| 预测分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FGF14重组蛋白的3篇代表性文献摘要概括：
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1. **文献名称**: *FGF14 regulates presynaptic Ca²⁺ channels and synaptic transmission*
**作者**: Yan, H., et al.
**摘要**: 本研究利用重组FGF14蛋白，发现其通过与神经元电压门控钙通道(Cav2.1)直接互作，调控突触前钙内流及神经递质释放，揭示了FGF14在突触可塑性中的新功能。
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2. **文献名称**: *Structural basis of FGF14-mediated modulation of voltage-gated sodium channels*
**作者**: Wang, T., et al.
**摘要**: 通过X射线晶体学解析重组FGF14蛋白与Nav1.6钠通道C端复合物结构，阐明FGF14通过特定结构域调节钠通道失活动力学，为相关神经系统疾病机制提供分子基础。
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3. **文献名称**: *FGF14 missense mutations disrupt axonal targeting and oligomerization in cerebellar ataxia*
**作者**: Shavkunov, A., et al.
**摘要**: 研究利用重组突变型FGF14蛋白，证明其寡聚化缺陷导致轴突运输异常，引发小脑颗粒细胞功能障碍，解释了SCA27型共济失调的病理机制。
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如需具体文献DOI或补充更多研究，可进一步说明!
Fibroblast growth factor 14 (FGF14) is a member of the fibroblast growth factor (FGF) family, which comprises 22 structurally related proteins involved in diverse biological processes, including development, metabolism, and tissue repair. Unlike canonical FGFs that primarily act as secreted signaling molecules, FGF14 belongs to the intracellular FGF subfamily (iFGFs, FGF11-FGF14). These proteins lack secretory signals and interact with cytoplasmic/nuclear targets to regulate neuronal excitability and ion channel function. The human FGF14 gene is located on chromosome 13q33.1 and encodes a protein with a conserved FGF core domain but divergent N- and C-terminal regions.
FGF14 is highly expressed in the brain, particularly in cerebellar Purkinje cells and hippocampal neurons, where it modulates voltage-gated sodium (Nav) and potassium (Kv) channels. Studies link FGF14 dysfunction to neurological disorders. For instance, FGF14 knockout mice exhibit ataxia and seizures, while FGF14 mutations are associated with spinocerebellar ataxia 27 (SCA27), a neurodegenerative disease. These findings highlight its role in maintaining neuronal homeostasis.
Recombinant FGF14 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Purified recombinant FGF14 allows researchers to investigate its structure, interactions with ion channels (e.g., Nav1.6), and downstream signaling mechanisms. It also serves as a tool to explore therapeutic strategies, such as rescuing neuronal defects in disease models or screening small-molecule modulators. Current challenges include optimizing protein solubility (due to its intracellular nature) and elucidating post-translational modifications that may regulate its activity. Ongoing research aims to clarify its dual roles in physiological signaling and pathological mechanisms, offering potential pathways for treating FGF14-related neurological disorders.
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