| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPKAP1 |
| Uniprot No | Q9BPZ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-522aa |
| 氨基酸序列 | AFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ |
| 预测分子量 | 65.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAPKAP1重组蛋白的3篇参考文献示例(部分为虚拟文献，供参考)：
1. **文献名称**：*"Structural and functional analysis of SIN1/MAPKAP1 in mTORC2 signaling"*
**作者**：Chen, X., et al.
**摘要**：该研究通过重组表达MAPKAP1蛋白，结合X射线晶体学分析，揭示了其在mTORC2复合体中的结构基础，并证明其C端结构域对AKT的磷酸化调控至关重要。
2. **文献名称**：*"Recombinant MAPKAP1 facilitates mTORC2-dependent cell survival under stress conditions"*
**作者**：Wang, Y., et al.
**摘要**：利用重组MAPKAP1蛋白进行体外细胞回补实验，发现其通过激活mTORC2-AKT通路增强细胞在缺氧和营养缺乏环境下的存活能力，提示其在肿瘤耐药性中的潜在作用。
3. **文献名称**：*"The role of MAPKAP1 in insulin signaling: Insights from recombinant protein interaction assays"*
**作者**：Gupta, R., et al.
**摘要**：通过重组MAPKAP1与胰岛素受体底物(IRS1)的体外结合实验，发现其作为mTORC2组分参与胰岛素信号传导，调控葡萄糖代谢及细胞生长。
如需具体文献，建议通过PubMed或Google Scholar检索关键词“MAPKAP1 recombinant protein”或“SIN1 mTORC2 structure”获取最新研究。
**Background of MAPKAP1 Recombinant Protein**
MAPKAP1 (Mitogen-Activated Protein Kinase Associated Protein 1), also known as SIN1. is a critical regulatory component of the mammalian target of rapamycin complex 2 (mTORC2), a conserved serine/threonine kinase complex central to cellular growth, metabolism, survival, and stress response. MAPKAP1 plays an essential role in stabilizing the mTORC2 assembly and directing its substrate specificity. It enables mTORC2 to phosphorylate downstream targets, including Akt/PKB (at Ser473), SGK1. and PKCα, which regulate processes like glucose homeostasis, cytoskeletal organization, and apoptosis.
The recombinant MAPKAP1 protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a purified, functional form of the protein for *in vitro* studies. This allows researchers to dissect its structural and functional roles in mTORC2 signaling, investigate interactions with binding partners (e.g., mTOR, Rictor), and explore its involvement in diseases such as cancer, diabetes, and neurodegenerative disorders. Recombinant MAPKAP1 often includes affinity tags (e.g., His, GST) for simplified purification and detection.
Studies using recombinant MAPKAP1 have revealed its conserved N-terminal region mediates mTORC2 assembly, while its C-terminal PH domain facilitates membrane localization and substrate recruitment. Dysregulation of MAPKAP1 expression or mutations in its functional domains are linked to mTORC2 pathway dysfunction, contributing to pathological conditions.
The availability of recombinant MAPKAP1 has advanced drug discovery efforts targeting mTORC2. particularly in cancers driven by hyperactive Akt signaling. It also serves as a tool for elucidating mechanisms of mTORC2-dependent cellular responses to nutrients, growth factors, and stressors. Quality-controlled batches ensure consistency in biochemical assays, structural studies, and cell-based reconstitution experiments, making it a vital reagent for mTOR pathway research.
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