| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPC5L |
| Uniprot No | Q9BPX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-154aa |
| 氨基酸序列 | MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRHSITGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV |
| 预测分子量 | 16,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARPC5L重组蛋白的3篇参考文献示例(注：文献信息为示例，请根据实际研究验证和补充)：
1. **文献名称**: "ARPC5L regulates the Arp2/3 complex and cell migration through its interaction with actin filaments"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过重组ARPC5L蛋白表达及功能分析，揭示了其在Arp2/3复合体中的结构作用，证明ARPC5L通过调控肌动蛋白成核影响细胞迁移能力。
2. **文献名称**: "Structural insights into the role of ARPC5L in the assembly of the Arp2/3 complex"
**作者**: Smith J, et al.
**摘要**: 利用重组ARPC5L蛋白进行冷冻电镜分析，解析了其在Arp2/3复合体中的结合位点，阐明其如何稳定复合体构象以促进肌动蛋白分支形成。
3. **文献名称**: "ARPC5L deficiency impairs dendritic cell function via dysregulated actin polymerization"
**作者**: Lee H, et al.
**摘要**: 通过重组ARPC5L蛋白回补实验，发现ARPC5L缺失导致树突状细胞中Arp2/3活性下降，影响免疫突触形成和抗原呈递功能。
建议通过PubMed或Google Scholar以关键词“ARPC5L recombinant”或“ARPC5L Arp2/3”检索最新文献以获取准确信息。
**Background of ARPC5L Recombinant Protein**
ARPC5L (Actin-Related Protein 2/3 Complex Subunit 5-Like) is a component of the Arp2/3 complex, a conserved multi-protein assembly critical for regulating actin cytoskeleton dynamics. The Arp2/3 complex facilitates actin nucleation and branching, processes essential for cell motility, membrane protrusion, vesicular trafficking, and intracellular transport. Unlike its paralog ARPC5. ARPC5L is a less-studied isoform, though it shares structural homology and is implicated in modulating the complex’s stability and activity.
Research suggests ARPC5L may play specialized roles in certain cell types or contexts. For example, it is enriched in hematopoietic cells and linked to immune functions, such as T-cell activation and dendritic cell migration. Dysregulation of ARPC5L has been associated with pathological conditions, including cancers and immune disorders, highlighting its potential as a therapeutic target.
Recombinant ARPC5L protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to enable functional and structural studies. Its production allows researchers to investigate isoform-specific contributions to actin dynamics, protein-protein interactions within the Arp2/3 complex, and mechanisms underlying diseases. Additionally, recombinant ARPC5L serves as a tool for drug screening, antibody development, and deciphering signaling pathways linked to cytoskeletal remodeling.
Characterizing ARPC5L advances understanding of actin-driven cellular processes and may uncover novel strategies for targeting cytoskeleton-related pathologies. Further studies are needed to clarify its distinct biological roles compared to other Arp2/3 subunits and its broader implications in health and disease.
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