| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTFP1 |
| Uniprot No | Q9UDX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-166aa |
| 氨基酸序列 | MSEPQPRGAERDLYRDTWVRYLGYANEVGEAFRSLVPAAVVWLSYGVASSYVLADAIDKGKKAGEVPSPEAGRSARVTVAVVDTFVWQALASVAIPGFTINRVCAASLYVLGTATRWPLAVRKWTTTALGLLTIPIIIHPIDRSVDFLLDSSLRKLYPTVGKPSSS |
| 预测分子量 | 45.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTFP1重组蛋白的模拟参考文献示例(仅供参考，实际文献需根据具体研究查询)：
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1. **文献名称**: *"Recombinant MTFP1 promotes mitochondrial fission by modulating membrane dynamics in vitro"*
**作者**: Chen, L. et al.
**摘要**: 本研究成功在大肠杆菌系统中表达并纯化了重组MTFP1蛋白，证实其可通过与线粒体膜蛋白DRP1相互作用，增强线粒体分裂活性。实验表明重组MTFP1在体外能诱导脂质体膜结构重塑。
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2. **文献名称**: *"Functional characterization of MTFP1 knockout cells rescued by recombinant MTFP1 expression"*
**作者**: Smith, J.R. & Patel, K.
**摘要**: 通过慢病毒载体在MTFP1敲除的HeLa细胞中表达重组人源MTFP1蛋白，发现其可恢复线粒体形态异常并逆转细胞凋亡抵抗表型，提示MTFP1在维持线粒体稳态中的关键作用。
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3. **文献名称**: *"Optimization of MTFP1 recombinant protein production in mammalian cell lines"*
**作者**: Wang, Y. et al.
**摘要**: 比较了HEK293和CHO细胞中MTFP1重组蛋白的表达效率，优化了分泌表达条件，获得高纯度蛋白并验证其与ANT2的结合能力，为后续功能研究提供工具。
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4. **文献名称**: *"MTFP1 interacts with the mitochondrial permeability transition pore: Insights from recombinant protein crosslinking assays"*
**作者**: García-Sánchez, A. et al.
**摘要**: 利用重组MTFP1蛋白进行交联实验和共免疫沉淀，揭示其与线粒体通透性转换孔(mPTP)组分CYPD的直接互作，提示其在细胞应激响应中的调控机制。
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**注**：MTFP1(Mitochondrial Fission Process 1)的研究多集中于其在线粒体动态调控和细胞凋亡中的作用，实际文献需通过PubMed、Web of Science等平台以关键词“MTFP1 recombinant”“MTFP1 expression”检索。
**Background of MTFP1 Recombinant Protein**
MTFP1 (Mitochondrial Fission Process 1), also known as MTP18 or PGAM5-MTFP1. is a mitochondrial membrane-associated protein implicated in regulating mitochondrial dynamics, particularly mitochondrial fission. It plays a critical role in maintaining mitochondrial morphology and cellular energy homeostasis. MTFP1 interacts with key fission machinery components, such as Dynamin-related protein 1 (Drp1), to facilitate membrane scission. Dysregulation of MTFP1 has been linked to mitochondrial fragmentation, apoptosis, and pathologies including neurodegenerative diseases, cancer, and metabolic disorders.
Recombinant MTFP1 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cell lines) to express the purified, functional protein for research applications. Its production enables detailed studies on mitochondrial fission mechanisms, protein-protein interactions, and drug discovery targeting mitochondrial dysfunction. Researchers utilize MTFP1 recombinant protein in assays like pull-down experiments, structural analysis, and cellular studies to explore its role in stress responses, mitophagy, and disease pathways.
Recent studies highlight MTFP1 as a potential biomarker or therapeutic target, particularly in cancers where altered mitochondrial dynamics influence tumor progression. Its recombinant form provides a tool to dissect mitochondrial quality control pathways and develop interventions for conditions linked to impaired mitochondrial fission.
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