| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNB1L |
| Uniprot No | Q9BYB4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MTAPCPPPPPDPQFVLRGTQSPVHALHFCEGAQAQGRPLLFSGSQSGLVHIWSLQTRRAVTTLDGHGGQCVTWLQTLPQGRQLLSQGRDLKLCLWDLAEGRSAVVDSVCLESVGFCRSSILAGGQPRWTLAVPGRGSDEVQILEMPSKTSVCALKPKADAKLGMPMCLRLWQADCSSRPLLLAGYEDGSVVLWDVSEQKVCSRIACHEEPVMDLDFDSQKARGISGSAGKALAVWSLDWQQALQVRGTHELTNPGIAEVTIRPDRKILATAGWDHRIRVFHWRTMQPLAVLAFHSAAVQCVAFTADGLLAAGSKDQRISLWSLYPRA |
| 预测分子量 | 62.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNB1L重组蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**: *"Molecular Cloning and Characterization of GNB1L, a Novel Candidate Gene for 22q11.2 Deletion Syndrome"*
**作者**: Lindsay, E.A., et al.
**摘要**: 该研究首次克隆了GNB1L基因，并发现其位于22q11.2染色体区域(与威廉姆斯综合征相关)。通过重组蛋白表达技术，验证了GNB1L在HEK293细胞中的表达，并推测其可能通过参与G蛋白信号通路影响神经发育。
2. **文献名称**: *"Functional Analysis of GNB1L in Cardiac Development Using Recombinant Protein Interaction Assays"*
**作者**: Ishihara, K., et al.
**摘要**: 研究利用重组GNB1L蛋白进行体外结合实验，发现其与转录因子TBX1存在直接互作，可能通过调控心脏发育相关基因的表达参与先天性心脏病的发生，为22q11.2缺失综合征的病理机制提供新证据。
3. **文献名称**: *"Structural Insights into GNB1L and Its Role in Chromatin Remodeling Complexes"*
**作者**: Zhang, Y., et al.
**摘要**: 通过重组GNB1L蛋白的结晶和结构解析，揭示了其WD40结构域介导与染色质重塑复合物(如BAF)的结合，提示GNB1L可能通过表观遗传调控影响细胞分化及器官形成。
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**注**：以上文献信息为示例性概括，实际研究需根据具体数据库(如PubMed、Web of Science)检索。若需进一步分析，建议结合实验方向补充具体文献。
GNB1L (G Protein Subunit Beta 1 Like) is a protein-coding gene located within the 22q11.2 chromosomal region, a locus frequently associated with microdeletion disorders such as DiGeorge syndrome and velocardiofacial syndrome. These conditions are characterized by congenital heart defects, immune deficiencies, and neurodevelopmental abnormalities, implicating GNB1L as a potential contributor to their pathogenesis. The GNB1L protein shares structural homology with the β-subunit of heterotrimeric G proteins, though it lacks the characteristic WD40 repeats, suggesting a divergent functional role. It is believed to participate in protein-protein interactions, cellular signaling, and chromatin remodeling, potentially influencing transcriptional regulation and developmental processes.
Recombinant GNB1L protein is engineered for in vitro studies to elucidate its molecular functions and interactions. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein is purified via affinity tags (e.g., His-tag) to ensure high specificity and stability. Its applications span functional assays, structural studies, and interaction screens with partners such as chromatin modifiers (e.g., TRIM28) or components of the BAF complex, which regulate gene expression. Researchers also employ it to model 22q11.2 deletion-related pathologies, exploring mechanisms underlying neurodevelopmental defects or cardiac malformations. Additionally, recombinant GNB1L serves as an antigen for antibody development or in diagnostic platforms. By dissecting its role in cellular pathways, this tool advances understanding of disease mechanisms and therapeutic targeting for associated syndromes.
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