| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAF1 |
| Uniprot No | Q9H063 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-256aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKLLENSSFEAINSQLTVETGDAHIIGRIE SYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEE EGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNA VNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGED GSLWSFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVE EESRSRGSGAEETSTMEEDRVPVICI |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"MAF1 is an essential mediator of nutrient and hormone signaling in metabolic homeostasis"**
- **作者**: Yuan, Y., et al.
- **摘要**: 研究通过重组MAF1蛋白的体外实验，揭示了其在调控mTORC1信号通路中的作用，证明MAF1通过抑制RNA聚合酶III的活性参与能量代谢平衡和胰岛素敏感性调节，与肥胖及糖尿病相关。
2. **"Structural insights into the mechanism of MAF1-dependent repression of RNA polymerase III"**
- **作者**: Johnson, S.S., et al.
- **摘要**: 利用重组MAF1蛋白进行X射线晶体学分析，解析了其与RNA聚合酶III复合物的结合位点，阐明MAF1通过构象变化抑制转录起始的分子机制，并验证了磷酸化修饰对其功能的调控作用。
3. **"Recombinant MAF1 interacts with PTEN and modulates cancer cell proliferation"**
- **作者**: Lee, J., & Kim, D.H.
- **摘要**: 通过重组MAF1蛋白的体外互作实验，发现其与肿瘤抑制因子PTEN直接结合，协同抑制肿瘤细胞中RNA Pol III的转录活性，揭示了MAF1在癌症治疗中的潜在靶点价值。
4. **"MAF1 phosphorylation by CK2 regulates its nuclear localization and transcriptional repression activity"**
- **作者**: Krzysztof, W., et al.
- **摘要**: 研究使用重组MAF1蛋白进行激酶实验，证明CK2激酶介导的磷酸化调控MAF1的核质穿梭，进而影响其对RNA Pol III的抑制能力，为细胞周期与应激反应的联系提供了新证据。
(注：以上文献为示例，实际文献需根据具体数据库检索结果调整。)
MAF1 (MAF1 homolog, negative regulator of RNA polymerase III) is a conserved protein that acts as a global suppressor of RNA polymerase III (Pol III)-mediated transcription, a key process in synthesizing transfer RNAs (tRNAs), 5S rRNA, and other small non-coding RNAs. Initially identified in yeast, MAF1 homologs are found across eukaryotes, including humans. It integrates metabolic and stress signals to regulate Pol III activity, linking cellular energy status to biosynthetic output. Under nutrient-rich conditions, MAF1 is phosphorylated and inactive, allowing Pol III-driven transcription to support cell growth. Stressors like nutrient deprivation, DNA damage, or inhibition of the mTOR signaling pathway trigger MAF1 dephosphorylation, enabling its translocation to the nucleus. Activated MAF1 binds to Pol III and represses transcription, conserving resources during stress.
Recombinant MAF1 protein is engineered for in vitro studies to dissect its molecular interactions, post-translational modifications, and structural features. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it enables biochemical assays, crystallography, and drug screening. Research highlights MAF1's role beyond Pol III regulation, including potential involvement in cancer (via tRNA synthesis control), metabolic disorders, and longevity. Its ability to modulate translation efficiency and cellular stress responses makes it a target for therapeutic strategies. Recombinant MAF1 tools have advanced understanding of how transcriptional regulation interfaces with mTOR signaling, autophagy, and energy homeostasis. However, mechanistic details of its nuclear shuttling, binding partners, and tissue-specific functions remain active areas of investigation.
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