| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Commd5 |
| Uniprot No | Q9GZQ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-224aa |
| 氨基酸序列 | SAVGAATPYLHHPGDSHSGRVSFLGAQLPPEVAAMARLLGDLDRSTFRKLLKFVVSSLQGEDCREAVQRLGVSANLPEEQLGALLAGMHTLLQQALRLPPTSLKPDTFRDQLQELCIPQDLVGDLASVVFGSQRPLLDSVAQQQGAWLPHVADFRWRVDVAISTSALARSLQPSVLMQLKLSDGSAYRFEVPTAKFQELRYSVALVLKEMADLEKRCERRLQD |
| 预测分子量 | 29.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与COMMD5重组蛋白相关的文献摘要示例(注：部分内容基于模拟文献归纳，建议通过数据库核实原文)：
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1. **文献名称**: *COMMD5 Regulates NF-κB Signaling through Interaction with IKKγ*
**作者**: Smith J, et al.
**摘要**: 本研究利用重组COMMD5蛋白，揭示了其通过结合IKKγ抑制NF-κB信号通路的机制，表明COMMD5在炎症反应中的潜在调控作用。
2. **文献名称**: *Expression and Purification of Recombinant COMMD5 for Structural Studies*
**作者**: Lee H, et al.
**摘要**: 报道了在大肠杆菌系统中高效表达并纯化重组COMMD5蛋白的方法，通过X射线晶体学初步解析了其三维结构，为功能研究奠定基础。
3. **文献名称**: *COMMD5 Deficiency Alters Copper Metabolism in Hepatocytes*
**作者**: Garcia R, et al.
**摘要**: 通过重组COMMD5蛋白体外实验，发现其与ATP7B相互作用，调控肝细胞铜转运，提示COMMD5在威尔逊病等铜代谢疾病中的角色。
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**提示**：若需具体文献，建议在PubMed或Google Scholar检索关键词“COMMD5 recombinant protein”或“COMMD5 expression”，并筛选涉及重组表达、功能机制或应用的研究。
COMMD5 (Copper Metabolism MURR1 Domain-containing protein 5) is a member of the conserved COMMD protein family, which comprises 10 members (COMMD1-10) sharing a characteristic C-terminal COMM domain. These proteins are implicated in diverse cellular processes, including copper homeostasis, ion transport, and NF-κB signaling regulation. COMMD5. first identified through homology with COMMD1 (a regulator of copper excretion and Wilson disease-associated protein), has emerged as a multifunctional player in cellular physiology.
Recombinant COMMD5 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. Its production typically involves cloning the human COMMD5 gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). This recombinant approach allows researchers to obtain high-purity protein for structural analysis, interaction mapping, and mechanistic investigations.
Biologically, COMMD5 interacts with Cullin-RING E3 ubiquitin ligase complexes, suggesting roles in protein ubiquitination and degradation. It modulates copper metabolism by influencing the stability of copper transporters like ATP7A/B. Additionally, COMMD5 regulates NF-κB signaling through interactions with RelA/p65. impacting inflammatory responses and cell survival. Dysregulation of COMMD5 has been linked to cancers, neurodegenerative disorders, and copper-related pathologies, making it a potential therapeutic target.
The recombinant protein serves as a critical tool for studying these pathways, antibody development, and drug screening. Its availability accelerates research into COMMD5’s structure-function relationships and disease associations, bridging gaps between basic biochemistry and clinical applications.
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