| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLRT3 |
| Uniprot No | Q9NZU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-528aa |
| 氨基酸序列 | KSCPSVCRCD AGFIYCNDRF LTSIPTGIPE DATTLYLQNN QINNAGIPSD LKNLLKVERI YLYHNSLDEF PTNLPKYVKE LHLQENNIRT ITYDSLSKIP YLEELHLDDN SVSAVSIEEG AFRDSNYLRL LFLSRNHLST IPWGLPRTIE ELRLDDNRIS TISSPSLQGL TSLKRLVLDG NLLNNHGLGD KVFFNLVNLT ELSLVRNSLT AAPVNLPGTN LRKLYLQDNH INRVPPNAFS YLRQLYRLDM SNNNLSNLPQ GIFDDLDNIT QLILRNNPWY CGCKMKWVRD WLQSLPVKVN VRGLMCQAPE KVRGMAIKDL NAELFDCKDS GIVSTIQITT AIPNTVYPAQ GQWPAPVTKQ PDIKNPKLTK DHQTTGSPSR KTITITVKSV TSDTIHISWK LALPMTALRL SWLKLGHSPA FGSITETIVT GERSEYLVTA LEPDSPYKVC MVPMETSNLY LFDETPVCIE TETAPLRMYN PTTTLNREQE KEPYKNPNLP LEHHHHHH |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FLRT3重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Structural mechanisms of plexin-mediated FLRT3 recognition*
**作者**：Seiradake, E., et al.
**摘要**：该研究通过晶体结构分析揭示了FLRT3重组蛋白胞外结构域与plexin受体间的相互作用，阐明了FLRT3作为排斥性信号分子在神经轴突导向中的分子机制，并验证了重组FLRT3对细胞迁移的抑制作用。
2. **文献名称**：*FLRT3 interacts with Unc5B to regulate angiogenesis in zebrafish*
**作者**：Yamagishi, S., et al.
**摘要**：研究利用重组FLRT3蛋白进行体外结合实验，证实其与Unc5B受体特异性结合，并通过斑马鱼模型证明该互作在血管发育中的关键作用，揭示了FLRT3/Unc5B复合物调控内皮细胞迁移的路径。
3. **文献名称**：*FLRT3 functions as a tumor suppressor in breast cancer by disrupting Wnt/β-catenin signaling*
**作者**：Müller, T., et al.
**摘要**：文章通过表达重组FLRT3蛋白进行功能研究，发现其通过竞争性结合Wnt配体抑制β-catenin信号通路，从而抑制乳腺癌细胞增殖和转移，为FLRT3的抑癌机制提供了实验依据。
4. **文献名称**：*Recombinant FLRT3 decoy receptors modulate neuronal circuit formation*
**作者**：Leyva-Díaz, E., et al.
**摘要**：该研究设计重组FLRT3诱饵蛋白(胞外段Fc融合蛋白)，证明其通过干扰内源性FLRT3与神经受体间的相互作用，调控小鼠皮层神经元突触的可塑性和神经网络组装。
FLRT3 (Fibronectin Leucine-Rich Transmembrane Protein 3) is a member of the FLRT family, a group of cell adhesion proteins that play dual roles in mediating cell-cell interactions and modulating intracellular signaling pathways. Structurally, FLRT3 contains an extracellular domain with leucine-rich repeats (LRRs) and a fibronectin type III (FN3) domain, followed by a single-pass transmembrane region and a short cytoplasmic tail. These domains enable FLRT3 to act as both a receptor and a ligand, engaging in homophilic and heterophilic interactions with proteins like Unc5 receptors, latrophilins, and heparan sulfate proteoglycans.
Functionally, FLRT3 is critical during embryonic development, particularly in nervous system patterning, angiogenesis, and tissue morphogenesis. It regulates cell adhesion, migration, and axon guidance by modulating signaling cascades such as the Netrin/DCC and TGF-β pathways. FLRT3 also exhibits context-dependent roles in cancer, acting as a tumor suppressor or promoter depending on the cellular environment.
Recombinant FLRT3 protein, typically produced in mammalian or bacterial expression systems, retains the functional extracellular domain and is used as a research tool to study its biological activities. Purified via affinity tags (e.g., His-tag or Fc-fusion), it serves in cell-based assays, structural studies, and in vivo models to dissect its role in development and disease. Its therapeutic potential is being explored for conditions involving aberrant cell adhesion or signaling, such as neurological disorders and cancer metastasis.
Overall, FLRT3 recombinant protein provides a versatile platform for investigating cell communication mechanisms and developing targeted interventions in biomedicine.
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