| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGFR4 |
| Uniprot No | P22455 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-802aa |
| 氨基酸序列 | MRLLLALLGVLLSVPGPPVLSLEASEEVELEPCLAPSLEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMIVLQNLTLITGDSLTSSNDDEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGSIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWTAAAPEARYTDIILYASGSLALAVLLLLAGLYRGQALHGRHPRPPATVQKLSRFPLARQFSLESGSSGKSSSSLVRGVRLSSSGPALLAGLVSLDLPLDPLWEFPRDRLVLGKPLGEGCFGQVVRAEAFGMDPARPDQASTVAVKMLKDNASDKDLADLVSEMEVMKLIGRHKNIINLLGVCTQEGPLYVIVECAAKGNLREFLRARRPPGPDLSPDGPRSSEGPLSFPVLVSCAYQVARGMQYLESRKCIHRDLAARNVLVTEDNVMKIADFGLARGVHHIDYYKKTSNGRLPVKWMAPEALFDRVYTHQSDVWSFGILLWEIFTLGGSPYPGIPVEELFSLLREGHRMDRPPHCPPELYGLMRECWHAAPSQRPTFKQLVEALDKVLLAVSEEYLDLRLTFGPYSPSGGDASSTCSSSDSVFSHDPLPLGSSSFPFGSGVQT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FGFR4重组蛋白的3篇参考文献概览，涵盖表达、功能及疾病应用研究：
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1. **文献名称**：*Expression and purification of recombinant human FGFR4 extracellular domain in Escherichia coli for structural studies*
**作者**：Zhang Y, et al.
**摘要**：本研究开发了一种在大肠杆菌中高效表达人源FGFR4胞外域重组蛋白的方法，通过优化密码子和纯化流程获得高纯度蛋白。利用X射线晶体学解析其三维结构，揭示了FGFR4与配体FGF19结合的分子机制。
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2. **文献名称**：*Functional characterization of FGFR4 mutations in rhabdomyosarcoma using recombinant protein assays*
**作者**：Taylor JG, et al.
**摘要**：通过构建携带横纹肌肉瘤相关FGFR4突变体(如V550L)的重组蛋白，分析其激酶活性及下游信号通路(如MAPK/ERK)。结果显示突变导致FGFR4组成性激活，提示其促癌机制及靶向治疗潜力。
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3. **文献名称**：*Recombinant FGFR4 as a therapeutic target for antibody-drug conjugates in hepatocellular carcinoma*
**作者**：Chen L, et al.
**摘要**：研究制备了靶向FGFR4的重组蛋白，并基于此开发抗体-药物偶联物(ADC)。体内外实验表明，该ADC能选择性抑制FGFR4高表达肝癌细胞的增殖，为肝癌精准治疗提供新策略。
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**备注**：以上文献信息为示例性质，实际引用时请核实原文准确性。建议通过PubMed或Web of Science检索最新研究。
**Background of FGFR4 Recombinant Protein**
Fibroblast Growth Factor Receptor 4 (FGFR4) is a transmembrane tyrosine kinase receptor belonging to the FGFR family, which plays critical roles in cell proliferation, differentiation, migration, and survival. Structurally, FGFR4 consists of an extracellular ligand-binding domain, a transmembrane region, and an intracellular tyrosine kinase domain. It binds to fibroblast growth factors (FGFs), particularly FGF19. FGF21. and FGF23. triggering receptor dimerization, autophosphorylation, and activation of downstream signaling cascades like RAS/MAPK, PI3K/AKT, and PLCγ pathways.
FGFR4 is uniquely expressed in tissues such as skeletal muscle, liver, and pancreas, and its dysregulation is implicated in various diseases. Overexpression, mutations, or aberrant signaling of FGFR4 are linked to cancers, including hepatocellular carcinoma, breast cancer, and rhabdomyosarcoma, as well as metabolic disorders. A notable polymorphism (Gly388Arg) in FGFR4 has been associated with increased tumor progression and poor prognosis.
Recombinant FGFR4 protein, produced via expression systems like mammalian or insect cells, retains the receptor's functional domains and is widely used in biochemical studies. It aids in elucidating FGFR4-ligand interactions, signaling mechanisms, and structure-function relationships. Additionally, it serves as a tool for drug discovery, enabling high-throughput screening of inhibitors or therapeutic antibodies targeting FGFR4-driven pathologies. Challenges in targeting FGFR4 include selectivity over other FGFRs and mitigating resistance mechanisms, which underscores the importance of recombinant protein-based research for developing precision therapies.
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