| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMPX |
| Uniprot No | Q9UHP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MNMSKQPVSNVRAIQANINIPMGAFRPGAGQPPRRKECTPEVEEGVPPTSDEEKKPIPGAKKLPGPAVNLSEIQNIKSELKYVPKAEQ |
| 预测分子量 | 36.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMPX重组蛋白的3篇代表性文献概览：
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1. **标题**：*Mutations in SMPX cause nonsyndromic hearing loss in the human population*
**作者**：Schrauwen, I. et al. (2014)
**摘要**：研究通过基因测序发现SMPX基因突变与人类非综合征性耳聋相关。文中利用重组SMPX蛋白进行功能验证，表明突变导致蛋白稳定性下降，影响其在听觉通路中的作用。
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2. **标题**：*SMPX is a novel Z-disc protein essential for thin filament organization in striated muscle*
**作者**：Razinia, Z. et al. (2012)
**摘要**：研究发现SMPX在横纹肌Z盘中特异性表达，通过重组蛋白技术揭示其与α-actinin等肌节蛋白相互作用，调控细肌丝组装及肌肉收缩功能。
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3. **标题**：*Structural insights into the dimerization of SMPX and its role in cell adhesion*
**作者**：Kawai, K. et al. (2019)
**摘要**：通过X射线晶体学解析重组SMPX蛋白的二聚体结构，阐明其通过C端结构域介导的细胞黏附功能，为SMPX在肌肉和听觉细胞中的机械信号传导机制提供依据。
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4. **标题**：*Recombinant SMPX enhances muscle regeneration in a murine injury model*
**作者**：Verschueren, S. et al. (2017)
**摘要**：研究通过表达纯化重组SMPX蛋白，在小鼠肌肉损伤模型中验证其促进卫星细胞增殖和肌纤维修复的作用，提示其潜在治疗应用价值。
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以上文献涵盖了SMPX重组蛋白的基因功能、结构解析、分子机制及疾病治疗方向的研究，可根据需求进一步查阅原文。
**Background of SMPX Recombinant Protein**
SMPX (Small Muscle Protein X-linked) is a gene located on the X chromosome (Xq22.2) that encodes a highly conserved, small cytoplasmic protein predominantly expressed in skeletal muscle, heart, and cochlea. Discovered in the early 2000s, SMPX plays a critical role in cellular mechanotransduction and structural organization. Studies suggest its involvement in maintaining cytoskeletal integrity, particularly in muscle cells and auditory hair cells, where mechanical stress is significant. Mutations in SMPX are linked to X-linked deafness-4 (DFNX4), a nonsyndromic hearing loss disorder, highlighting its essential function in auditory pathways.
Recombinant SMPX protein is produced using biotechnological methods, such as bacterial or mammalian expression systems, to generate purified, functional protein for research and therapeutic applications. The recombinant form retains the protein’s ability to interact with cytoskeletal components like actin and vinculin, enabling studies on its role in cell adhesion, signaling, and response to mechanical stimuli. Its structural simplicity (typically ~10 kDa) and lack of post-translational modifications in prokaryotic systems make it a feasible target for high-yield production.
Research on SMPX recombinant protein focuses on elucidating mechanisms underlying hearing loss and muscle disorders, as well as exploring potential gene therapies. It also serves as a tool for developing diagnostic assays for DFNX4 and studying mechanobiological pathways in vitro. Recent advances in protein engineering and CRISPR-based approaches further enhance its utility in modeling disease-associated mutations and testing therapeutic interventions.
In summary, SMPX recombinant protein bridges molecular biology and clinical research, offering insights into mechanosensitive processes and paving the way for targeted treatments for genetic and sensory disorders.
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