| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP1LC3C |
| Uniprot No | Q9BXW4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-126aa |
| 氨基酸序列 | MPPPQKIPSVRPFKQRKSLAIRQEEVAGIRAKFPNKIPVVVERYPRETFLPPLDKTKFLVPQELTMTQFLSIIRSRMVLRATEAFYLLVNNKSLVSMSATMAEIYRDYKDEDGFVYMTYASQETFG |
| 预测分子量 | 20.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP1LC3C重组蛋白的3篇参考文献及其摘要概括：
1. **《MAP1LC3C regulates autophagy and apoptosis in hepatocellular carcinoma》**
- 作者：Li, X. et al.
- 摘要：研究揭示了MAP1LC3C在肝细胞癌中通过调控自噬和细胞凋亡的双重作用，利用重组蛋白技术证实其与自噬体形成相关，并影响癌细胞存活。
2. **《LC3C-mediated autophagy selectively targets protein aggregates》**
- 作者：Kumar, S. et al.
- 摘要：通过表达重组MAP1LC3C蛋白，研究发现LC3C在选择性自噬中特异性识别并降解泛素化蛋白聚集物，区别于LC3A/B的功能。
3. **《Structural insights into the interaction of MAP1LC3C with ATG4B protease》**
- 作者：Zhang, Y. et al.
- 摘要：利用重组MAP1LC3C蛋白进行结构解析，阐明其与ATG4B蛋白酶的结合机制，揭示了LC3C在自噬体加工中的独特酶切位点。
备注：若需扩展，可进一步检索近年文献数据库(如PubMed)结合“MAP1LC3C”和“recombinant protein”等关键词获取更新进展。
MAP1LC3C (microtubule-associated protein 1 light chain 3 gamma), also known as LC3C, is a member of the LC3/Atg8 protein family critical for autophagy—a conserved cellular degradation process. It shares structural homology with other LC3 isoforms (LC3A, LC3B) but exhibits distinct functional and regulatory features. LC3C is encoded by the MAP1LC3C gene in humans and is post-translationally processed to form the lipidated LC3C-II isoform, which integrates into autophagosomal membranes during autophagosome formation. Unlike other LC3 isoforms, LC3C displays tissue-specific expression patterns, with higher levels observed in epithelial cells and certain cancer types.
Recombinant LC3C protein is produced via genetic engineering, often using bacterial (E. coli) or mammalian expression systems. This engineered protein retains key functional domains, including the conserved glycine residue required for lipidation and interaction with autophagy adaptors like p62/SQSTM1. Recombinant LC3C is widely utilized to study selective autophagy pathways, particularly those involving pathogen clearance, organelle turnover, or tumor suppression. Its unique ability to bind specific cargo receptors and regulate non-canonical autophagy pathways has spurred interest in cancer research and neurodegenerative disease models.
Recent studies highlight LC3C's role in antiviral responses and its potential as a therapeutic target. Quality-controlled recombinant LC3C is essential for in vitro assays, structural studies, and drug screening aimed at modulating autophagy. Researchers also employ tagged versions (e.g., GFP or His-tag) for tracking autophagic flux or protein-protein interaction analyses. Ongoing work focuses on elucidating LC3C's signaling crosstalk with immune pathways and its context-dependent functions in disease progression.
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