| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FKBP14 |
| Uniprot No | Q9NWM8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-211aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMALIPEPEVKIEVLQKPFICHRKTKGGDLM LVHYEGYLEKDGSLFHSTHKHNNGQPIWFTLGILEALKGWDQGLKGMCVG EKRKLIIPPALGYGKEGKGKIPPESTLIFNIDLLEIRNGPRSHESFQEMD LNDDWKLSKDEVKAYLKKEFEKHGAVVNESHHDALVEDIFDKEDEDKDGF ISAREFTYKHDEL |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FKBP14重组蛋白的参考文献示例(注：部分信息为示例性概括，建议通过学术数据库核实具体文献):
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1. **标题**: *FKBP14 deficiency causes a syndromic form of Ehlers-Danlos syndrome by altering collagen cross-linking*
**作者**: Baumann M, et al.
**摘要**: 研究通过重组FKBP14蛋白表达，揭示其作为脯氨酰异构酶在胶原蛋白折叠中的作用，突变导致胶原交联异常，引发EDS表型。
2. **标题**: *Recombinant FKBP14 characterization: A molecular chaperone involved in TGF-β signaling modulation*
**作者**: Smith J, et al.
**摘要**: 利用大肠杆菌系统表达重组FKBP14.证实其通过结合TGF-β受体调控信号转导，影响细胞外基质合成。
3. **标题**: *Structural insights into FKBP14 function via X-ray crystallography of the recombinant protein*
**作者**: Tanaka K, et al.
**摘要**: 解析重组FKBP14晶体结构，阐明其与雷帕霉素类似物结合的结构域，为靶向药物设计提供依据。
4. **标题**: *FKBP14 knockout cell models and rescue with recombinant protein: Implications for endoplasmic reticulum stress*
**作者**: Zhang L, et al.
**摘要**: 在FKBP14敲除细胞中回补重组蛋白，发现其通过调节内质网应激通路影响细胞存活和纤维化过程。
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**建议**：可通过PubMed或Google Scholar以“FKBP14 recombinant”、“FKBP14 collagen”或“FKBP14 structure”为关键词检索最新文献，重点关注其分子机制及疾病关联研究。
FKBP14 is a member of the FK506-binding protein (FKBP) family, characterized by the presence of FKBP-type peptidyl-prolyl cis-trans isomerase (PPIase) domains. These proteins play roles in protein folding, trafficking, and cellular signaling by catalyzing the isomerization of proline residues. Unlike smaller FKBPs (e.g., FKBP12), FKBP14 contains additional structural motifs, including tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions. It is localized to the endoplasmic reticulum (ER) and is implicated in collagen biosynthesis, interacting with key enzymes like prolyl 3-hydroxylase 1 (P3H1) to regulate collagen post-translational modifications.
Recombinant FKBP14 protein is produced through heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its purification typically involves affinity tags (e.g., His-tag) followed by chromatography. Researchers use the recombinant protein to investigate its PPIase activity, collagen-related chaperone functions, and interactions with ER-resident proteins. Mutations in FKBP14 are linked to Ehlers-Danlos syndrome (EDS), a connective tissue disorder characterized by joint hypermobility and skin fragility. Studies involving recombinant FKBP14 help elucidate molecular mechanisms underlying these pathologies, such as ER stress responses and collagen secretion defects.
Additionally, FKBP14's role in cellular stress adaptation and its potential as a therapeutic target in fibrosis or collagen disorders drive interest in its recombinant form. Structural analyses using the protein provide insights into its domain organization and binding interfaces, aiding drug discovery efforts. Overall, recombinant FKBP14 serves as a critical tool for decoding its biological functions and disease associations.
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