| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NLE1 |
| Uniprot No | Q9NVX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-485aa |
| 氨基酸序列 | AAAVPDEAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRLQLVCNALLAQEDPLPLAFFVHDAEIVSSLGKTLESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKGHRHWVLSISWSPDGRKLASGCKNGQILLWDPSTGKQVGRTLAGHSKWITGLSWEPLHANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVWDVKAQKLAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR |
| 预测分子量 | 69.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NLE1重组蛋白的3篇参考文献的简要信息(注：文献为虚拟示例，实际需根据具体研究补充)：
1. **文献名称**：*"Cloning and Functional Characterization of the Escherichia coli Effector Protein Nle1"*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中克隆并表达了重组NLE1蛋白，证实其通过抑制宿主NF-κB信号通路参与免疫逃逸，为致病机制研究提供工具。
2. **文献名称**：*"Structural Analysis of Recombinant Nle1 Reveals a Novel Interaction with Host Cell Ubiquitination Machinery"*
**作者**：Chen L, et al.
**摘要**：通过重组NLE1蛋白的结晶和结构解析，揭示了其与宿主泛素化酶复合物的特异性结合，阐明其在调控炎症反应中的作用机制。
3. **文献名称**：*"Optimization of Nle1 Recombinant Protein Expression and Purification for High-Throughput Screening"*
**作者**：Wang X, et al.
**摘要**：优化了NLE1蛋白在原核系统中的可溶性表达条件，建立高效纯化流程，为基于NLE1的抑制剂筛选平台奠定基础。
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**备注**：实际研究中建议通过PubMed或Google Scholar以关键词“Nle1 recombinant protein”或“Nle1 expression”检索最新文献，重点关注病原体-宿主相互作用或蛋白生化特性相关研究。
**Background of NLE1 Recombinant Protein**
NLE1 (Non-Lethal Effector Protein 1) is a bacterial effector protein primarily associated with enteropathogenic *Escherichia coli* (EPEC) and enterohemorrhagic *E. coli* (EHEC), which are pathogens responsible for severe gastrointestinal diseases. These bacteria employ a type III secretion system (T3SS) to inject effector proteins, including NLE1. directly into host cells. Once delivered, NLE1 manipulates host cellular processes to subvert immune responses, promote bacterial survival, and facilitate infection.
Studies have shown that NLE1 plays a critical role in disrupting host innate immune signaling pathways, particularly the NF-κB pathway, which is central to inflammation and antimicrobial defense. NLE1 achieves this by interacting with host proteins involved in ubiquitination and proteasomal degradation, effectively dampening pro-inflammatory signals. Its ability to interfere with immune surveillance highlights its importance in bacterial pathogenesis.
Recombinant NLE1 protein is produced using genetic engineering techniques, typically through expression in *E. coli* or other heterologous systems, followed by purification. This engineered protein retains the functional domains necessary for studying its molecular mechanisms, such as binding assays, structural analysis, or host-pathogen interaction experiments. Researchers utilize recombinant NLE1 to investigate its role in infection models, screen for inhibitors, or develop diagnostic tools.
The study of NLE1 recombinant protein provides insights into bacterial virulence strategies and host immune evasion mechanisms. It also serves as a potential target for therapeutic interventions aimed at neutralizing pathogenic effects during EPEC/EHEC infections. Continued research on NLE1 may contribute to novel antimicrobial strategies or vaccines targeting T3SS-dependent pathogens.
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