| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCF7L2 |
| Uniprot No | Q9NQB0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 氨基酸序列 | MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTLHFQSGSTHYSAYKTIEHQIAVQYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWLVPQQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKRDKQPGETNEHSECFLNPCLSLPPIT |
| 预测分子量 | 54.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCF7L2重组蛋白的3篇代表性文献摘要(模拟示例，非真实文献)：
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1. **文献名称**: *Structural basis of TCF7L2 recognition by β-catenin in the Wnt signaling pathway*
**作者**: Beato, M., et al.
**摘要**: 本研究解析了重组TCF7L2蛋白与β-catenin复合物的晶体结构(3.2 Å)，揭示了二者结合的分子界面，并发现TCF7L2的N端结构域对Wnt信号激活至关重要，为糖尿病相关突变位点提供结构解释。
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2. **文献名称**: *Functional characterization of TCF7L2 variants in type 2 diabetes using recombinant protein assays*
**作者**: Grant, S.F., et al.
**摘要**: 通过重组表达人类TCF7L2蛋白，结合荧光素酶报告基因实验，发现rs7903146风险等位基因导致TCF7L2转录活性降低，影响胰岛素分泌相关基因(如GLP-1)的表达调控。
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3. **文献名称**: *Recombinant TCF7L2 DNA-binding domain production and interaction with chromatin modifiers*
**作者**: Staal, F.J., et al.
**摘要**: 利用大肠杆菌系统高效表达并纯化TCF7L2的DNA结合域(重组蛋白)，通过体外Pull-down实验证实其与组蛋白乙酰转移酶p300的相互作用，提示表观遗传调控在结直肠癌中的作用机制。
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注：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以“TCF7L2 recombinant protein”为关键词检索最新论文。真实文献可能涉及重组蛋白在基因编辑(CRISPR筛选)、药物靶点筛选或类器官模型中的应用。
TCF7L2 (T-cell factor 7-like 2) is a transcription factor encoded by the *TCF7L2* gene on human chromosome 10q25.3. It plays a pivotal role in the Wnt/β-catenin signaling pathway, a conserved pathway regulating cell proliferation, differentiation, and metabolic homeostasis. TCF7L2 acts as a nuclear mediator, binding to β-catenin to activate target genes involved in pancreatic development, insulin secretion, and glucose metabolism. Notably, genome-wide association studies identified *TCF7L2* as the strongest genetic risk factor for type 2 diabetes (T2D), with specific single-nucleotide polymorphisms (SNPs) significantly increasing susceptibility. Dysregulation of TCF7L2 is also implicated in colorectal cancer, where aberrant Wnt signaling drives tumorigenesis.
Recombinant TCF7L2 protein is engineered to study its molecular interactions, structural features, and functional roles in vitro. Produced via bacterial (e.g., *E. coli*) or mammalian expression systems, the protein typically includes functional domains like the N-terminal β-catenin-binding region and the C-terminal high-mobility group (HMG) DNA-binding domain. Tags such as His-tag or GST facilitate purification and detection. Researchers use recombinant TCF7L2 to investigate its binding affinity with β-catenin, DNA, or co-regulators (e.g., CREB-binding protein), as well as to screen small-molecule inhibitors targeting Wnt signaling in diabetes or cancer. Structural studies (e.g., X-ray crystallography) reveal conformational changes during complex formation. Additionally, recombinant proteins enable functional validation of disease-associated variants (e.g., rs7903146) via electrophoretic mobility shift assays or luciferase reporter systems. Its application extends to developing diagnostic tools and therapeutic strategies, emphasizing its dual role in metabolic and oncogenic pathways. Purified TCF7L2 retains DNA-binding activity, validated by in vitro assays, making it a critical reagent for mechanistic studies in molecular biology and translational research.
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