| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FZR1 |
| Uniprot No | Q9UM11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 氨基酸序列 | MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPASSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPDDGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNAEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLFTRIR |
| 预测分子量 | 70.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FZR1重组蛋白的3条参考文献示例(注：以下内容为示例性概括，实际文献需根据具体研究检索)：
1. **文献名称**：*"Structural and functional characterization of recombinant FZR1 as a regulator of the anaphase-promoting complex"*
**作者**：Chen et al., 2017
**摘要**：本研究通过大肠杆菌表达系统纯化重组FZR1蛋白，分析其与APC/C复合物的相互作用，揭示FZR1在细胞周期退出和G1期维持中的关键作用，并证明其磷酸化修饰对功能的影响。
2. **文献名称**：*"FZR1 recombinant protein enhances ubiquitination in vitro: Implications for cancer therapy"*
**作者**：Kimura et al., 2020
**摘要**：利用昆虫细胞表达体系获得高纯度FZR1重组蛋白，证明其作为APC/C的共激活因子可促进特定底物泛素化降解，并探讨其在抑制肿瘤细胞增殖中的潜在应用。
3. **文献名称**：*"Development of a novel FZR1 recombinant protein-based assay for detecting dysregulation in neurodegenerative diseases"*
**作者**：Gupta et al., 2019
**摘要**：构建FZR1重组蛋白体外检测平台，发现其在阿尔茨海默病模型中与异常蛋白聚集体的相关性，为神经退行性疾病机制研究提供新工具。
如需具体文献，建议通过PubMed或Web of Science以“FZR1 recombinant protein”“APC/C-FZR1”等关键词检索。
FZR1 (Fizzy-related protein 1), also known as Cdh1. is a regulatory subunit of the anaphase-promoting complex/cyclosome (APC/C), a critical ubiquitin ligase governing cell cycle progression. It plays a central role in coordinating the transition from mitosis to G1 phase by targeting specific substrates for proteasomal degradation, including cyclins and other cell cycle inhibitors. Unlike its homolog CDC20. which activates APC/C during early mitosis, FZR1 is essential for maintaining the G1 state and enabling proper cell differentiation, DNA repair, and metabolic homeostasis. Its activity is tightly regulated through phosphorylation and interactions with other signaling pathways.
Recombinant FZR1 protein is engineered using expression systems like *E. coli* or mammalian cell cultures to produce purified, functional protein for biochemical studies. This engineered form retains key domains, including the conserved C-terminal IR motif and substrate-binding sites, allowing researchers to investigate its binding partners, enzymatic dynamics, and regulatory mechanisms. Researchers often utilize it in *in vitro* ubiquitination assays, structural studies (e.g., cryo-EM), or high-throughput screens to identify modulators of APC/C activity.
Dysregulation of FZR1 is implicated in cancer, neurodegeneration, and developmental disorders. For instance, its loss correlates with genomic instability in tumors, while overexpression may disrupt neuronal connectivity. Recombinant FZR1 enables exploration of these disease links, offering insights into cell cycle-targeted therapies. Recent studies also highlight its non-canonical roles in autophagy and metabolism, expanding its therapeutic relevance. By leveraging recombinant FZR1. scientists aim to decode its multifunctional biology and develop precision interventions for cell cycle-related pathologies.
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