| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R3B |
| Uniprot No | Q9Y5P8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-575aa |
| 氨基酸序列 | MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSGLEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVNVDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNCHDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGRITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTKMIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLANVFFDTFFNIEKYLDHEQKEQISLLRDGDSGGPELSDWEKYAAEEYDILVAEETAGEPWEDGFEAELSPVEQKLSALRSPLAQRPFFEAPSPLGAVDLYEYACGDEDLEPL |
| 预测分子量 | 72.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PPP2R3B重组蛋白的模拟参考文献(实际文献需通过PubMed等数据库查询)：
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1. **文献名称**: *Structural and Functional Characterization of PPP2R3B as a Regulatory Subunit of Protein Phosphatase 2A*
**作者**: Smith J, et al.
**摘要**: 本研究解析了PPP2R3B重组蛋白的结构，证明其通过与PP2A催化亚基结合调控磷酸酶活性，并参与细胞周期检查点调控。
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2. **文献名称**: *PPP2R3B Dysregulation Promotes Tumor Metastasis via AKT Signaling Pathway*
**作者**: Chen L, et al.
**摘要**: 发现PPP2R3B在多种癌症中表达异常，通过重组蛋白实验证实其可通过AKT通路影响肿瘤细胞迁移和侵袭，提示其作为潜在治疗靶点。
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3. **文献名称**: *PPP2R3B Recombinant Protein Restores Neuronal Function in Alzheimer’s Disease Models*
**作者**: Gonzalez R, et al.
**摘要**: 利用重组PPP2R3B蛋白干预阿尔茨海默病模型，发现其通过调节tau蛋白过度磷酸化改善神经元功能障碍，为神经退行性疾病提供新思路。
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**注**：以上为模拟文献，实际研究请参考权威数据库(如PubMed、Google Scholar)或联系领域专家获取准确信息。
**Background of PPP2R3B Recombinant Protein**
PPP2R3B (Protein Phosphatase 2 Regulatory Subunit B'' Beta) is a regulatory component of the protein phosphatase 2A (PP2A) complex, a major serine/threonine phosphatase critical for cellular processes such as cell cycle regulation, signal transduction, and apoptosis. As part of the B'' subfamily, PPP2R3B functions as a scaffold to recruit specific substrates and modulate PP2A’s enzymatic activity, ensuring precise dephosphorylation events. Its structure typically includes conserved HEAT repeats, which facilitate protein-protein interactions and subcellular localization.
Recombinant PPP2R3B is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified protein for functional studies. Researchers utilize it to investigate PP2A’s regulatory mechanisms, particularly its role in diseases linked to phosphatase dysregulation, such as cancer, neurodegenerative disorders, and metabolic syndromes. For example, PPP2R3B has been implicated in tumor suppression pathways, where its loss or mutation may disrupt PP2A-mediated control of oncogenic kinases.
Studies also explore PPP2R3B’s interaction with viral proteins, highlighting its involvement in host-pathogen interactions. The recombinant form enables structural analysis (e.g., X-ray crystallography) and biochemical assays to map binding interfaces or screen therapeutic compounds targeting PP2A-associated pathways.
Production typically involves cloning the PPP2R3B gene into expression vectors, followed by affinity chromatography (e.g., His-tag purification). Quality assessments include SDS-PAGE, Western blotting, and activity assays. As a research tool, PPP2R3B recombinant protein advances understanding of PP2A’s diverse roles and potential therapeutic applications in precision medicine.
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