| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTMR7 |
| Uniprot No | Q9Y216 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660aa |
| 氨基酸序列 | MEHIRTPKVENVRLVDRVSPKKAALGTLYLTATHVIFVENSPDPRKETWILHSQISTIEKQATTATGCPLLIRCKNFQIIQLIIPQERDCHDVYISLIRLARPVKYEELYCFSFNPMLDKEEREQGWVLIDLSEEYTRMGLPNHYWQLSDVNRDYRVCDSYPTELYVPKSATAHIIVGSSKFRSRRRFPVLSYYYKDNHASICRSSQPLSGFSARCLEDEQMLQAIRKANPGSDFVYVVDTRPKLNAMANRAAGKGYENEDNYSNIKFQFIGIENIHVMRNSLQKMLEVCELKSPSMSDFLWGLENSGWLRHIKAIMDAGIFIAKAVSEEGASVLVHCSDGWDRTAQVCSVASLLLDPHYRTLKGFMVLIEKDWISFGHKFNHRYGNLDGDPKEISPVIDQFIECVWQLMEQFPCAFEFNERFLIHIQHHIYSCQFGNFLCNSQKERRELKIQERTYSLWAHLWKNRADYLNPLFRADHSQTQGTLHLPTTPCNFMYKFWSGMYNRFEKGMQPRQSVTDYLMAVKEETQQLEEELEALEERLEKIQKVQLNCTKVKSKQSEPSKHSGFSTSDNSIANTPQDYSGNMKSFPSRSPSQGDEDSALILTQDNLKSSDPDLSANSDQESGVEDLSCRSPSGGEHAPSEDSGKDRDSDEAVFLTA |
| 预测分子量 | 91.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTMR7重组蛋白的3篇参考文献示例(内容基于公开文献概括，非真实文献，仅供参考)：
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1. **文献名称**: *"Characterization of Recombinant MTMR7 Phosphatase Activity and Its Role in Autophagy Regulation"*
**作者**: Smith A, et al.
**摘要**: 本研究成功表达并纯化了人源MTMR7重组蛋白，证实其具有特异性磷酸酶活性，可水解PtdIns3P和PtdIns(3.5)P2.实验表明MTMR7通过与自噬相关蛋白Beclin-1相互作用，负调控细胞自噬过程。
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2. **文献名称**: *"Structural Insights into MTMR7 Dimerization Mediated by Coiled-Coil Domains"*
**作者**: Zhang L, et al.
**摘要**: 通过X射线晶体学解析了MTMR7重组蛋白的晶体结构，发现其C端卷曲螺旋结构域介导二聚化。研究揭示了二聚化对MTMR7磷酸酶活性和亚细胞定位的关键作用，为疾病相关突变分析提供结构基础。
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3. **文献名称**: *"MTMR7 Knockdown Enhances Axon Regeneration via PI3K/AKT Signaling"*
**作者**: Chen R, et al.
**摘要**: 利用重组MTMR7蛋白进行体外功能实验，发现其通过抑制PI3K/AKT通路抑制神经轴突再生。在MTMR7缺失模型中，PI3P水平升高促进神经元损伤修复，提示其作为神经退行性疾病的潜在治疗靶点。
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(注：以上文献为示例性内容，实际研究中请通过PubMed或学术数据库检索真实文献。)
MTMR7 (Myotubularin-related protein 7) is a member of the myotubularin family of lipid phosphatases, which specifically dephosphorylate phosphatidylinositol (PI) signaling molecules, particularly PI3P and PI(3.5)P2. These lipids play critical roles in membrane trafficking, autophagy, and endocytic pathways. MTMR7 is characterized by a conserved catalytic active site within its protein tyrosine phosphatase (PTP) domain, along with a PH-GRAM domain for membrane association and a coiled-coil domain mediating protein interactions. Unlike some family members, MTMR7 lacks enzymatic activity unless it forms heterodimers with catalytically active partners like MTMR1 or MTMR2. suggesting a regulatory role in phosphoinositide metabolism.
Recombinant MTMR7 protein is engineered for in vitro studies to investigate its structural properties, substrate specificity, and interaction networks. Its production typically involves expression systems (e.g., E. coli or mammalian cells) followed by purification via affinity tags. Research highlights its involvement in neuronal development and maintenance, as mutations in related myotubularins are linked to Charcot-Marie-Tooth neuropathy. Dysregulation of MTMR7 has also been implicated in cancer progression, potentially through PI3K/AKT/mTOR pathway modulation. Additionally, studies using recombinant MTMR7 explore its role in autophagy-lysosomal dysfunction, a key feature in neurodegenerative diseases. This protein serves as a tool for drug screening aimed at targeting lipid phosphatase activity or restoring phosphoinositide homeostasis in pathological conditions.
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