| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | fap2 |
| Uniprot No | Q9UTM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-433aa |
| 氨基酸序列 | VELAKNHSFDNIIAIDAEPVPSSMSAANDINKIVRPEYADLKYMKLALEAMEKWRNDPELSSVYFECGRLSTISKDPYRARFDEVAQRNLRKLLGDSALINLSSSEEIRKKYPSLFSNSPLRSDMQAVVNEHAGYANSAASLKLLELKARELGVEFVFGKAGKFKKFVVNHSETDIDKNDNHVSVQTEDGTIYHADTILLAVGAYLNAYLNTSHRVCAKGLPVAHIQLTDEEFKTYKNMPIIFDPDCAYAFPPYPVTKLIKLASTGYEYVCNVETDYDENSKVVSIPHSGPSKSSLPKYAIIQMRRFLDTFLPDLADRSLINTKMCWISDTEDANFLIDKVPQFDNVFVANGDSGHAFKFLPNIGRYIAQRILGDLSEEWKDAWRWREDDKASELKWRCVRSLIDYKDAEFTYDK |
| 预测分子量 | 52.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Fusobacterium nucleatum **Fap2蛋白**的3篇参考文献及其摘要概述：
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1. **文献名称**：*Fap2 mediates Fusobacterium nucleatum colorectal adenocarcinoma enrichment by binding to tumor-expressed Gal-GalNAc*
**作者**：Gur C. et al.
**摘要**：该研究揭示了具核梭杆菌的Fap2蛋白通过识别宿主细胞表面的半乳糖/N-乙酰半乳糖胺(Gal-GalNAc)结构，介导细菌在结直肠癌组织中的特异性富集。重组Fap2蛋白实验证明其结合Gal-GalNAc的能力，解释了该菌在肿瘤微环境中的选择性定植机制。
2. **文献名称**：*Fap2 of Fusobacterium nucleatum is a galactose-inhibitable lectin involved in coaggregation and immune evasion*
**作者**：Coppenhagen-Glazer S. et al.
**摘要**：研究发现Fap2蛋白作为半乳糖敏感的凝集素，介导具核梭杆菌与其他口腔细菌的共聚集，并抑制自然杀伤(NK)细胞的细胞毒性。重组Fap2实验表明其通过结合NK细胞表面受体(如TIGIT)抑制免疫反应，促进细菌免疫逃逸。
3. **文献名称**：*Bacterial genotoxin accelerates transient cell cycle arrest and inhibits host DNA damage response*
**作者**：Abed J. et al.
**摘要**：文章探讨具核梭杆菌通过Fap2蛋白与结直肠癌细胞互作，激活DNA损伤信号通路并抑制宿主DNA修复机制。重组Fap2的体外实验显示其直接干扰细胞周期调控，促进癌前病变微环境的形成。
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**备注**：上述文献均围绕具核梭杆菌Fap2蛋白的功能展开，涉及肿瘤微环境调控、免疫逃逸及宿主互作机制。如需成纤维细胞激活蛋白(FAPα)相关研究，请进一步说明。
Fap2 (Fibroblast activation protein alpha) is a type II transmembrane glycoprotein belonging to the serine protease family, initially identified for its overexpression in activated fibroblasts within tumor microenvironments. It shares structural homology with dipeptidyl peptidase 4 (DPP4) and exhibits dual enzymatic activities: post-proline dipeptidyl peptidase and collagenase functions. These activities enable Fap2 to degrade extracellular matrix (ECM) components and regulate bioactive peptides, facilitating tissue remodeling and cell signaling modulation.
In cancer biology, Fap2 is implicated in tumor progression, immunosuppression, and metastasis. Stromal fibroblasts expressing Fap2 promote tumor growth by ECM degradation, releasing growth factors, and suppressing antitumor immune responses. Its restricted expression in healthy tissues (primarily wound healing sites) versus widespread presence in tumors makes it a potential therapeutic target.
Recombinant Fap2 proteins are engineered to study its biological roles and develop targeted therapies. Produced via heterologous expression systems (e.g., mammalian cells, insect cells), these proteins retain enzymatic activity and are used for structural studies, inhibitor screening, and antibody development. Notably, Fap2 inhibitors and Fap2-targeted CAR-T cells are under investigation for cancer treatment.
Recent studies also explore Fap2's role in fibrotic diseases and chronic inflammation, expanding its biomedical relevance. However, challenges remain in understanding its substrate specificity and regulatory mechanisms. Recombinant Fap2 tools continue to drive translational research, bridging mechanistic insights with clinical applications.
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