| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTL7A |
| Uniprot No | Q9Y615 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435aa |
| 氨基酸序列 | MWAPPAAIMGDGPTKKVGNQAPLQTQALQTASLRDGPAKRAVWVRHTSSEPQEPTESKAAKERPKQEVTKAVVVDLGTGYCKCGFAGLPRPTHKISTTVGKPYMETAKTGDNRKETFVGQELNNTNVHLKLVNPLRHGIIVDWDTVQDIWEYLFRQEMKIAPEEHAVLVSDPPLSPHTNREKYAEMLFEAFNTPAMHIAYQSRLSMYSYGRTSGLVVEVGHGVSYVVPIYEGYPLPSITGRLDYAGSDLTAYLLGLLNSAGNEFTQDQMGIVEDIKKKCCFVALDPIEEKKVPLSEHTIRYVLPDGKEIQLCQERFLCSEMFFKPSLIKSMQLGLHTQTVSCLNKCDIALKRDLMGNILLCGGSTMLSGFPNRLQKELSSMCPNDTPQVNVLPERDSAVWTGGSILASLQGFQPLWVHRFEYEEHGPFFLYRRCF |
| 预测分子量 | 64.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACTL7A重组蛋白的模拟参考文献示例(注：以下内容为虚构，仅用于演示格式)：
1. **"ACTL7A重组蛋白在精子发生中的功能研究"**
*作者：Li et al., 2018*
摘要：研究通过在大肠杆菌中表达ACTL7A重组蛋白，制备特异性抗体并分析其在精子形成中的作用，发现ACTL7A缺陷导致小鼠精子顶体结构异常。
2. **"人源ACTL7A重组蛋白的纯化及结构表征"**
*作者：Zhang et al., 2020*
摘要：利用昆虫细胞表达系统获得高纯度ACTL7A重组蛋白，通过X射线晶体学解析其三维结构，揭示其与肌动蛋白家族其他成员的差异性结合位点。
3. **"ACTL7A突变体的重组表达与男性不育关联分析"**
*作者：Wang et al., 2019*
摘要：构建ACTL7A突变体重组蛋白模型，体外实验表明特定突变(如p.R34H)破坏蛋白与SPATA3的相互作用，可能导致精子变形障碍。
4. **"ACTL7A重组蛋白在卵母细胞成熟中的调控机制"**
*作者：Chen et al., 2021*
摘要：通过体外重组蛋白递送实验，证实ACTL7A通过调节细胞骨架动态变化影响卵母细胞纺锤体组装，为生殖障碍提供新靶点。
(注：实际文献需通过PubMed、Google Scholar等平台以关键词“ACTL7A recombinant protein”检索。)
**Background of ACTL7A Recombinant Protein**
ACTL7A (Actin-like 7A) is a member of the actin-related protein (ARP) family, which shares structural homology with conventional actins but exhibits distinct functional roles. It is primarily expressed in the testis and plays a critical role in spermatogenesis, particularly during spermiogenesis—the phase where haploid spermatids mature into functional sperm. ACTL7A is essential for acrosome formation, nuclear shaping, and cytoplasmic reorganization, processes vital for sperm motility and fertility. Mutations or dysregulation of ACTL7A have been linked to male infertility, underscoring its biological significance.
Recombinant ACTL7A protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms for research and therapeutic applications. Its production typically involves cloning the ACTL7A gene into expression vectors, followed by protein purification via affinity chromatography (e.g., His-tag systems). The recombinant protein retains functional domains necessary for interacting with cytoskeletal components, enabling studies on its role in cellular dynamics, fertilization mechanisms, and potential contributions to reproductive disorders.
In research, ACTL7A recombinant protein is utilized to investigate molecular pathways in spermatogenesis, screen for infertility-related biomarkers, and develop diagnostic or therapeutic strategies for male reproductive health. It also serves as an antigen for antibody production or a reference in structural studies to elucidate its unique actin-like properties. Beyond reproduction, emerging studies explore its expression in cancers, suggesting broader implications in cell proliferation and metastasis. Overall, ACTL7A recombinant protein is a vital tool for advancing reproductive biology and translational medicine.
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