| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FARSA |
| Uniprot No | Q9Y285 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-508aa |
| 氨基酸序列 | ADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLDAEPRPPPTQEAA |
| 预测分子量 | 73.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FARSA重组蛋白的3-4篇示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库获取)：
1. **标题**：*"Recombinant Expression and Functional Characterization of Human Phenylalanyl-tRNA Synthetase Alpha Subunit (FARSA)"*
**作者**：Chen L, Wang Y, et al.
**摘要**：该研究报道了人源FARSA蛋白在大肠杆菌系统中的重组表达与纯化，并通过体外酶活实验验证其催化苯丙氨酸与tRNA结合的活性，为后续结构功能研究奠定基础。
2. **标题**：*"Structural Insights into FARSA's Role in tRNA Recognition by X-ray Crystallography"*
**作者**：Smith J, Tanaka R, et al.
**摘要**：作者解析了FARSA与tRNA复合物的晶体结构(分辨率2.8Å)，揭示了其催化结构域的关键氨基酸残基如何特异性识别tRNA，并提出了底物结合机制模型。
3. **标题**：*"Disease-associated Mutations in FARSA Disrupt Mitochondrial Protein Synthesis"*
**作者**：Gupta S, Patel K, et al.
**摘要**：研究通过重组野生型与突变型FARSA蛋白的功能比较，发现某些遗传性神经疾病相关的FARSA突变会导致线粒体tRNA苯丙酰化效率下降，进而影响线粒体翻译。
4. **标题**：*"A High-throughput Screening Assay for FARSA Inhibitors Using Recombinant Protein"*
**作者**：Kim H, Zhang X, et al.
**摘要**：开发了基于重组FARSA蛋白的荧光偏振高通量筛选平台，用于发现潜在抗菌或抗增殖化合物，并初步鉴定了两类小分子抑制剂。
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**备注**：以上文献为领域知识模拟示例，实际研究中建议通过PubMed、Web of Science或Google Scholar检索关键词(如：FARSA recombinant, Phenylalanyl-tRNA synthetase expression)获取真实文献。
FARSA, also known as phenylalanyl-tRNA synthetase alpha chain, is a key enzyme in the translation machinery responsible for catalyzing the attachment of phenylalanine to its cognate tRNA during protein synthesis. As a member of the aminoacyl-tRNA synthetase (ARS) family, FARSA operates in a heterotetrameric complex with FARSB (beta subunit) to ensure fidelity in genetic code translation. Structurally, it contains conserved catalytic domains for amino acid activation and tRNA binding, along with unique motifs that facilitate subunit interaction and proofreading.
Recombinant FARSA protein is produced through biotechnological methods, typically using expression systems like *E. coli* or mammalian cells. This engineered protein retains native enzymatic activity while offering advantages such as high purity, batch consistency, and customizable tags (e.g., His-tag) for simplified purification and detection. Its recombinant form enables precise studies of ARS functionality, including mechanistic insights into substrate recognition, ATP-dependent aminoacylation, and quality control processes.
Beyond canonical translation roles, emerging research highlights FARSA's "moonlighting" functions in cellular signaling, inflammation modulation, and angiogenesis. Dysregulation of FARSA has been linked to neurodevelopmental disorders, cancer metastasis, and autoimmune conditions, making it a potential biomarker or therapeutic target. Recombinant FARSA serves as a critical tool for drug screening (e.g., ARS inhibitors), structural biology (crystallography/Cryo-EM), and elucidating pathogenic mutations in ARS-related diseases. Recent studies also explore its role in immune response regulation through tRNA fragment generation, expanding its relevance to antiviral mechanisms and immunotherapies.
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