| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL14 |
| Uniprot No | P50914 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-214aa |
| 氨基酸序列 | VFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEVKKLQKAALLKASPKKAPGTKGTAAAAAAAAAAKVPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPKASGKK |
| 预测分子量 | 50.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL14重组蛋白的3篇参考文献示例(文献内容为虚构，仅供格式参考)：
1. **文献名称**：*Functional characterization of recombinant RPL14 in ribosome biogenesis*
**作者**：Chen L, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化RPL14重组蛋白，证实其与28S rRNA的结合能力，并揭示其在核糖体大亚基组装中的关键作用。
2. **文献名称**：*RPL14 interacts with c-Myc to promote tumor cell proliferation*
**作者**：Wang Y, et al.
**摘要**：利用重组RPL14蛋白进行免疫共沉淀实验，发现其与转录因子c-Myc直接互作，调控下游靶基因表达，促进结直肠癌细胞增殖。
3. **文献名称**：*Structural analysis of human RPL14 using recombinant protein crystallization*
**作者**：Kim S, et al.
**摘要**：通过昆虫细胞系统表达重组RPL14蛋白，解析其晶体结构，揭示了C端结构域在核糖体RNA识别中的构象变化机制。
(注：以上文献为示例，实际文献需通过数据库如PubMed检索。)
**Background of RPL14 Recombinant Protein**
RPL14. a member of the ribosomal protein L14E family, is a critical component of the 60S ribosomal subunit involved in protein synthesis. As a structural and functional element of ribosomes, RPL14 plays a role in mRNA binding, ribosome assembly, and translational fidelity. Beyond its canonical role in translation, RPL14 has been implicated in extra-ribosomal functions, including cell proliferation, differentiation, and apoptosis, with dysregulation linked to cancers (e.g., colorectal cancer, leukemia) and developmental disorders.
Recombinant RPL14 protein is engineered using biotechnological methods, often expressed in *E. coli* or mammalian systems (e.g., HEK293 cells) to ensure proper folding and post-translational modifications. The protein is purified via affinity tags (e.g., His-tag) and validated through techniques like SDS-PAGE, Western blotting, and mass spectrometry. Its production enables functional studies, such as investigating ribosome biogenesis, protein-RNA interactions, and mechanisms underlying RPL14-associated pathologies.
Research applications include elucidating RPL14's role in diseases, screening therapeutic targets, and studying ribosomopathies—disorders caused by ribosomal dysfunction. Additionally, recombinant RPL14 serves as an antigen for antibody development or diagnostic tools. Recent studies also explore its potential as a biomarker for cancer prognosis.
Overall, RPL14 recombinant protein is a vital tool for advancing molecular biology and translational research, bridging gaps between ribosomal biology, disease mechanisms, and therapeutic innovation.
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