| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS25 |
| Uniprot No | P62851 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA |
| 预测分子量 | 20.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS25重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*RPS25 binds to HCV IRES and enhances viral translation initiation*
**作者**：Stone, S. et al.
**摘要**：该研究揭示了RPS25作为核糖体40S亚基的关键蛋白，通过与丙型肝炎病毒(HCV)内部核糖体进入位点(IRES)结合，促进病毒RNA的翻译起始，为靶向RPS25的抗病毒治疗提供了依据。
2. **文献名称**：*CRISPR screen identifies RPS25 as a synthetic lethal target in MYC-driven cancers*
**作者**：Guo, J. et al.
**摘要**：通过CRISPR筛选发现，RPS25在MYC高表达的癌细胞中具有合成致死效应。抑制RPS25可选择性阻断肿瘤细胞增殖，表明其作为癌症治疗靶点的潜力。
3. **文献名称**：*Structural insights into RPS25-mediated ribosome stalling during stress response*
**作者**：Zhang, L. et al.
**摘要**：利用冷冻电镜解析了RPS25重组蛋白在核糖体应激停滞状态下的结构，揭示了其通过构象变化调控翻译暂停的分子机制，为理解细胞应激反应的调控提供了新视角。
4. **文献名称**：*RPS25-dependent ribosome heterogeneity regulates SARS-CoV-2 replication*
**作者**：Wang, Y. et al.
**摘要**：研究发现，新冠病毒(SARS-CoV-2)依赖宿主RPS25蛋白形成特殊核糖体亚群以高效翻译病毒RNA，敲低RPS25可显著抑制病毒复制，提示其作为抗病毒靶标的可能性。
注：以上文献信息为示例，具体内容需根据实际研究调整。
**Background of RPS25 Recombinant Protein**
RPS25 (Ribosomal Protein S25) is a component of the 40S ribosomal subunit, playing a critical role in mRNA translation and ribosome biogenesis. As part of the eukaryotic ribosome, it contributes to the structural integrity of the small subunit and facilitates the decoding of genetic information during protein synthesis. The protein is highly conserved across species, underscoring its fundamental role in cellular function.
Recent studies have highlighted RPS25’s involvement in specialized translation mechanisms. For instance, it is essential for the initiation of internal ribosome entry site (IRES)-mediated translation, a process utilized by certain viruses (e.g., hepatitis C virus) and stress-responsive cellular mRNAs to bypass canonical cap-dependent translation. This unique role has sparked interest in RPS25 as a potential therapeutic target for viral infections and cancers that rely on IRES-driven oncogene expression.
The recombinant RPS25 protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling high-purity yields for functional studies. Its recombinant form allows researchers to explore ribosome assembly, translation regulation, and interactions with viral or cellular IRES elements. Additionally, RPS25 has been implicated in cellular stress responses, apoptosis, and cell cycle regulation, linking its dysregulation to diseases like cancer and neurodegeneration.
Emerging CRISPR-based screens have further identified RPS25 as a critical host factor for SARS-CoV-2 replication, suggesting broader implications in viral pathogenesis. Ongoing research aims to dissect its molecular mechanisms and explore its utility in biotechnology and medicine.
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