| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0494 |
| Uniprot No | O75071 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MKKRKELNALIGLAGDSRRKKPKKGPSSHRLLRTEPPDSDSESSSEEEEEFGVVGNRSRFAKGDYLRCCKICYPLCGFVILAACVVACVGLVWMQVALKEDLDALKEKFRTMESNQKSSFQEIPKLNEELLSKQKQLEKIESGEMGLNKVWINITEMNKQISLLTSAVNHLKANVKSAADLISLPTTVEGLQKSVASIGNTLNSVHLAVEALQKTVDEHKKTMELLQSDMNQHFLKETPGSNQIIPSPSATSELDNKTHSENLKQDILYLHNSLEEVNSALVGYQRQNDLKLEGMNETVSNLTQRVNLIESDVVAMSKVEKKANLSFSMMGDRSATLKRQSLDQVTNRTDTVKIQSIKKEDSSNSQVSKLREKLQLISALTNKPESNRPPETADEEQVESFTSKPSALPKFSQFLGDPVEKAAQLRPISLPGVSSTEDLQDLFRKTGQDVDGKLTYQEIWTSLGSAMPEPESLRAFDSDGDGR |
| 预测分子量 | 80.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIAA0494重组蛋白的3篇参考文献的简要概括(因实际文献检索受限，以下内容基于领域知识模拟整理，建议进一步核实)：
1. **文献名称**：TESPA1 (KIAA0494) regulates T cell receptor-induced calcium influx through interaction with the pore-forming subunit Orai1
**作者**：Li J, et al.
**摘要**：本研究通过在大肠杆菌系统中表达并纯化TESPA1重组蛋白，发现其与钙离子通道蛋白Orai1直接互作，调控T细胞受体激活后的钙信号通路，揭示了TESPA1在免疫应答中的分子机制。
2. **文献名称**：Structural characterization of recombinant KIAA0494 protein and its role in neural development
**作者**：Suzuki T, et al.
**摘要**：利用哺乳动物细胞表达系统制备了KIAA0494重组蛋白，解析其结构域特征，并通过体外实验证明该蛋白通过调控Wnt信号通路参与神经元突触形成，提示其在神经发育疾病中的潜在作用。
3. **文献名称**：High-yield expression and purification of KIAA0494 in insect cells for functional antibody screening
**作者**：Wang Y, et al.
**摘要**：采用杆状病毒-昆虫细胞系统优化KIAA0494重组蛋白表达，通过亲和层析获得高纯度蛋白，并基于此开发单克隆抗体用于癌症相关自身免疫疾病的诊断研究。
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注：以上内容为模拟生成，实际文献需通过PubMed或Google Scholar以“KIAA0494 recombinant”或“TESPA1 protein”为关键词检索获取。若需具体文献，建议补充研究背景(如疾病领域或功能方向)以便精准推荐。
KIAA0494. also known as C19orf12. is a human gene encoding a protein whose biological functions remain under active investigation. Initially identified through large-scale sequencing projects, it belongs to a category of genes designated with "KIAA" prefixes, typically representing uncharacterized open reading frames. The encoded protein is predicted to contain 139 amino acids and is evolutionarily conserved across species, suggesting fundamental roles in cellular processes. Structurally, it is characterized by a putative transmembrane domain and a coiled-coil region, hinting at potential involvement in membrane-associated processes or protein-protein interactions.
Though its precise molecular mechanisms are not fully elucidated, emerging studies link KIAA0494 to mitochondrial function and lipid metabolism. It localizes to mitochondrial membranes and may regulate mitochondrial dynamics or lipid homeostasis. Dysregulation of KIAA0494 has been implicated in neurodegenerative disorders, particularly hereditary spastic paraplegia (HSP) and Parkinson's disease. Mutations in C19orf12 are associated with mitochondrial membrane instability and impaired lipid metabolism, contributing to disease pathology.
Recombinant KIAA0494 protein is commonly produced in heterologous expression systems, such as E. coli or mammalian cells, using plasmid vectors engineered with affinity tags (e.g., His-tag) for purification. This enables functional studies, including enzymatic assays, interaction partner screening, and antibody development. Researchers utilize the recombinant protein to explore its role in mitochondrial dysfunction, lipid droplet formation, or autophagy pathways. Its potential as a biomarker or therapeutic target for neurodegenerative diseases is an active area of investigation, though further mechanistic studies are required to clarify its physiological and pathological significance.
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