| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpsF |
| Uniprot No | P02358 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-131aa |
| 氨基酸序列 | MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANETADDAEAGDSEE |
| 预测分子量 | 42.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rpsF重组蛋白的3条参考文献示例(基于公开研究领域常见内容概括，具体文献需根据实际检索确认)：
1. **标题**：*Expression and Purification of Recombinant RpsF in Escherichia coli for Structural Studies*
**作者**：Smith A, et al.
**摘要**：研究报道了通过克隆rpsF基因到大肠杆菌表达系统，优化诱导条件并利用亲和层析技术纯化重组RpsF蛋白，为后续X射线晶体学研究提供高纯度蛋白样品。
2. **标题**：*Functional Characterization of Recombinant RpsF in Bacterial Ribosome Assembly*
**作者**：Chen L, et al.
**摘要**：通过体外重构实验验证重组RpsF蛋白在核糖体亚基组装中的功能，证明其与16S rRNA的相互作用对核糖体稳定性至关重要，为细菌蛋白质合成机制提供新见解。
3. **标题**：*Development of a RpsF-Specific Antibody Using Recombinant Protein Immunization*
**作者**：Wang Y, et al.
**摘要**：利用重组RpsF蛋白免疫小鼠制备多克隆抗体，验证其特异性并应用于检测细菌内源性RpsF表达水平，为病原体诊断工具开发奠定基础。
**注意**：以上内容为基于领域研究的示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议使用关键词“rpsF recombinant protein”或“ribosomal protein S6 expression”进一步查找。
The rpsF gene encodes the ribosomal protein S6. a critical component of the 30S subunit in prokaryotic ribosomes. This protein plays a pivotal role in translation initiation and mRNA binding, contributing to the structural stability and functional dynamics of the ribosome. In Escherichia coli, S6 is part of the "Shine-Dalgarno domain," interacting with 16S rRNA and facilitating the recognition of mRNA start sites. Its conservation across bacterial species underscores its essential role in protein synthesis, making it a subject of interest in studies on ribosome assembly, antibiotic resistance, and bacterial physiology.
Recombinant rpsF protein is produced via genetic engineering, typically by cloning the rpsF gene into expression vectors (e.g., pET or pGEX systems) and expressing it in host systems like E. coli. This approach allows large-scale production of the protein for structural and functional analyses. Purification methods often involve affinity chromatography, leveraging tags such as His-tag or GST-tag for efficient isolation.
Research on recombinant S6 has provided insights into ribosome biogenesis and interactions with antibiotics targeting translation machinery. For example, studies have explored how S6 mutations affect ribosome function or confer resistance to drugs like aminoglycosides. Additionally, structural analyses (e.g., X-ray crystallography, cryo-EM) of recombinant S6 have elucidated its role in ribosome subunit association and conformational changes during translation. Beyond basic science, this protein serves as a tool for developing novel antimicrobials or synthetic biology applications, highlighting its dual significance in fundamental and applied microbiology.
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