| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOSL2 |
| Uniprot No | P15408 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMYQDYPG NFDTSSRGSS GSPAHAESYS SGGGGQQKFR VDMPGSGSAF IPTINAITTS QDLQWMVQPT VITSMSNPYP RSHPYSPLPG LASVPGHMAL PRPGVIKTIG TTVGRRRRDE QLSPEEEEKR RIRRERNKLA AAKCRNRRRE LTEKLQAETE ELEEEKSGLQ KEIAELQKEK EKLEFMLVAH GPVCKISPEE RRSPPAPGLQ PMRSGGGSVG AVVVKQEPLE EDSPSSSSAG LDKAQRSVIK PISIAGGFYG EEPLHTPIVV TSTPAVTPGT SNLVFTYPSV LEQESPASPS ESCSKAHRRS SSSGDQSSDS LNSPTLLAL |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOSL2重组蛋白的3篇参考文献示例(注：文献为虚构示例，实际引用需核实真实来源)：
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1. **文献名称**: *"Recombinant FOSL2 Protein Expression and Its Role in Epithelial-Mesenchymal Transition"*
**作者**: Wang, Y. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化重组FOSL2蛋白，验证其促进肿瘤细胞上皮-间质转化(EMT)的功能，证明FOSL2通过激活MMP9通路增强癌细胞侵袭能力。
2. **文献名称**: *"Structural Characterization of FOSL2 Recombinant Protein and Its Dimerization with JUNB"*
**作者**: Müller, R. et al.
**摘要**: 利用X射线晶体学解析FOSL2重组蛋白结构，揭示其与JUNB形成异源二聚体的关键结合域，为靶向AP-1复合物的药物设计提供结构基础。
3. **文献名称**: *"FOSL2 Recombinant Protein Modulates TGF-β Signaling in Fibrotic Disease Models"*
**作者**: Chen, L. et al.
**摘要**: 通过体外实验证实重组FOSL2蛋白与Smad3相互作用，增强TGF-β信号传导，提示FOSL2可能在纤维化疾病中作为潜在治疗靶点。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词：**"FOSL2 recombinant protein"、"FOSL2 purification"** 或 **"FOSL2 functional analysis"**，并筛选近5年高影响力期刊文章。
FOSL2 (FOS-like antigen 2), also known as FRA2. is a member of the FOS family of transcription factors that form the activator protein 1 (AP-1) complex by dimerizing with JUN family proteins. This complex regulates gene expression by binding to specific DNA sequences, influencing cellular processes such as proliferation, differentiation, apoptosis, and stress responses. FOSL2 is structurally characterized by a basic leucine zipper (bZIP) domain critical for DNA binding and protein-protein interactions. Unlike other FOS members (e.g., c-FOS), FOSL2 exhibits distinct expression patterns and regulatory roles, often linked to developmental processes, tissue remodeling, and pathological conditions like cancer, fibrosis, and inflammation.
Recombinant FOSL2 protein is engineered using molecular cloning techniques, where the FOSL2 gene is expressed in host systems (e.g., E. coli, mammalian cells) to produce purified, functional protein. This recombinant form retains biological activity, enabling researchers to study its interactions, post-translational modifications, and downstream signaling pathways in vitro or in cell-based assays. Applications include investigating FOSL2's role in epithelial-mesenchymal transition (EMT), tumor progression, and extracellular matrix regulation. Its dysregulation in diseases like triple-negative breast cancer and pulmonary fibrosis highlights its therapeutic relevance. Recombinant FOSL2 also serves as a tool for screening inhibitors or modulators targeting AP-1-driven pathways, offering insights into potential treatment strategies. Strict quality controls ensure batch consistency, making it valuable for both basic research and drug discovery.
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