| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZSCAN21 |
| Uniprot No | Q9Y5A6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461aa |
| 氨基酸序列 | MMTKVLGMAPVLGPRPPQEQVGPLMVKVEEKEEKGKYLPSLEMFRQRFRQFGYHDTPGPREALSQLRVLCCEWLRPEIHTKEQILELLVLEQFLTILPQELQAWVQEHCPESAEEAVTLLEDLERELDEPGHQVSTPPNEQKPVWEKISSSGTAKESPSSMQPQPLETSHKYESWGPLYIQESGEEQEFAQDPRKVRDCRLSTQHEESADEQKGSEAEGLKGDIISVIIANKPEASLERQCVNLENEKGTKPPLQEAGSKKGRESVPTKPTPGERRYICAECGKAFSNSSNLTKHRRTHTGEKPYVCTKCGKAFSHSSNLTLHYRTHLVDRPYDCKCGKAFGQSSDLLKHQRMHTEEAPYQCKDCGKAFSGKGSLIRHYRIHTGEKPYQCNECGKSFSQHAGLSSHQRLHTGEKPYKCKECGKAFNHSSNFNKHHRIHTGEKPYWCHHCGKTFCSKSNLSK |
| 预测分子量 | 56.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZSCAN21重组蛋白的参考文献示例(注：内容为模拟生成，实际文献需通过学术数据库查询确认)：
1. **《Recombinant ZSCAN21 Protein Expression and DNA Binding Characterization》**
- **作者**: Zhang L, et al.
- **摘要**: 研究利用大肠杆菌系统成功表达并纯化ZSCAN21重组蛋白，通过电泳迁移实验(EMSA)证实其特异性结合靶DNA序列，为后续功能研究提供基础。
2. **《Structural Insights into ZSCAN21’s Role in Transcriptional Regulation》**
- **作者**: Wang Y, et al.
- **摘要**: 通过X射线晶体学解析ZSCAN21重组蛋白的锌指结构域三维结构，揭示其与染色质修饰复合物的相互作用机制，提示其在胚胎干细胞多能性维持中的作用。
3. **《Functional Analysis of ZSCAN21 Recombinant Protein in Neural Differentiation》**
- **作者**: Smith J, et al.
- **摘要**: 在哺乳动物细胞中表达ZSCAN21重组蛋白，发现其通过调控神经发育相关基因(如SOX2)的表达，影响体外神经干细胞的定向分化过程。
4. **《ZSCAN21 Recombinant Protein Interacts with HDACs and Modulates Epigenetic Landscapes》**
- **作者**: Li X, et al.
- **摘要**: 研究利用免疫共沉淀(Co-IP)技术证明ZSCAN21重组蛋白与组蛋白去乙酰化酶(HDACs)结合，可能通过表观遗传重编程参与癌症细胞的增殖抑制。
建议通过PubMed或Google Scholar检索真实文献，关键词如“ZSCAN21 recombinant protein”、“ZSCAN21 structure/function”等。
**Background of ZSCAN21 Recombinant Protein**
ZSCAN21 (Zinc Finger and SCAN Domain-Containing Protein 21) is a transcription factor belonging to the C2H2-type zinc finger protein family, characterized by its SCAN domain, which mediates protein-protein interactions, and multiple zinc finger motifs that bind DNA. It plays a regulatory role in gene expression, particularly in processes like cell differentiation, proliferation, and embryonic development. Studies suggest ZSCAN21 is involved in neurodevelopment, stem cell maintenance, and oncogenesis, though its precise mechanisms remain under investigation.
Recombinant ZSCAN21 protein is engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. This allows scientists to study its DNA-binding properties, interaction partners, and regulatory functions in controlled settings. Its recombinant form often includes tags (e.g., His-tag) for easy purification and detection.
Interest in ZSCAN21 stems from its potential roles in disease. For example, aberrant expression has been linked to cancers, where it may act as an oncogene or tumor suppressor depending on context. Additionally, its involvement in neuronal development highlights relevance to neurological disorders. Recombinant ZSCAN21 enables *in vitro* assays, structural studies, and screening for therapeutic modulators.
Despite progress, challenges persist in fully elucidating its target genes and tissue-specific roles. Ongoing research aims to clarify its dual functions in health and disease, leveraging recombinant tools to explore its therapeutic potential.
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