| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Acaca |
| Uniprot No | Q13085 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 78-617aa |
| 氨基酸序列 | SMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLI |
| 预测分子量 | 67.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Acaca(乙酰辅酶A羧化酶α)重组蛋白的示例参考文献，供参考：
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1. **文献名称**：*Crystal structure of yeast acetyl-CoA carboxylase complex with the antifungal agent soraphen A*
**作者**：Tong L., Harwood HJ Jr.
**摘要**：本研究解析了酵母来源的Acaca重组蛋白与其抑制剂soraphen A的复合物晶体结构，揭示了该酶的催化机制及药物结合位点，为基于结构的药物设计提供了依据。
2. **文献名称**：*Expression and purification of recombinant human acetyl-CoA carboxylase 2 in E. coli for biochemical characterization*
**作者**：Kim CW, Moon YA.
**摘要**：报道了人源Acaca重组蛋白在大肠杆菌中的高效表达与纯化策略，并通过酶动力学实验验证其催化活性，为体外研究脂肪酸代谢提供了工具。
3. **文献名称**：*Functional analysis of ACCα mutations in cancer using recombinant protein systems*
**作者**：Brusselmans K, De Schrijver E, Verhoeven G.
**摘要**：通过构建Acaca突变体重组蛋白，揭示了特定癌症相关突变对其酶活性和细胞脂质合成的影响，提示Acaca在肿瘤代谢中的调控作用。
4. **文献名称**：*Engineering thermostable acetyl-CoA carboxylase for improved microbial biofuel production*
**作者**：Liu X, Sheng J, Curtiss R.
**摘要**：利用定向进化技术改造Acaca重组蛋白，提高其热稳定性及催化效率，为微生物合成生物燃料的优化提供了新策略。
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**说明**：以上文献为模拟示例，实际引用时建议通过PubMed、Google Scholar等平台检索关键词(如"recombinant Acaca protein"、"ACCα expression")获取最新研究。
Acetyl-CoA carboxylase alpha (ACACA), also known as ACC1. is a critical enzyme in lipid metabolism. It catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid biosynthesis. This reaction not only provides substrates for fatty acid elongation but also regulates mitochondrial fatty acid oxidation through malonyl-CoA-mediated inhibition of carnitine palmitoyltransferase-1 (CPT1). ACACA exists as two isoforms in mammals: ACC1 (cytosolic) and ACC2 (mitochondrial-associated), with ACC1 being predominant in lipogenic tissues like liver and adipose.
Recombinant ACACA protein is typically produced using expression systems such as E. coli, insect cells, or mammalian cell cultures to enable functional studies. The recombinant form preserves the enzyme's multidomain structure, including biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), and carboxyltransferase (CT) domains, which are essential for its catalytic activity. Researchers often utilize affinity tags (e.g., His-tag) for purification and detection.
Studies employing recombinant ACACA have revealed its regulatory mechanisms, including phosphorylation by AMP-activated protein kinase (AMPK) and allosteric modulation by citrate. Dysregulation of ACACA has been implicated in metabolic disorders, cancer progression, and insulin resistance, making it a potential therapeutic target. Current applications of recombinant ACACA span enzyme kinetics analysis, inhibitor screening for metabolic disease treatment, and structural biology investigations to develop isoform-specific inhibitors. Its role in cancer metabolism has gained particular attention, as many tumors show upregulated ACACA expression to support membrane biosynthesis during rapid proliferation.
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