| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MT1F |
| Uniprot No | P04733 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-59aa |
| 氨基酸序列 | MDPNCSCAAGVSCTCAGSCKCKECKCTSCKKSCCSCCPVGCSKCAQGCVCKGASEKCSC |
| 预测分子量 | 7.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MT1F重组蛋白的模拟参考文献示例(注：文献信息为假设性概括，建议通过学术数据库检索真实文献)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of Human Metallothionein-1F in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人源MT1F蛋白，并通过亲和层析纯化获得高纯度产物。实验表明，重组MT1F对镉离子(Cd²⁺)具有强结合能力，并在细胞氧化应激模型中表现出显著的抗氧化活性，提示其在重金属解毒和氧化损伤修复中的潜在作用。
2. **文献名称**: "MT1F Overexpression Suppresses Tumor Metastasis via Epithelial-Mesenchymal Transition Inhibition in Gastric Cancer"
**作者**: Wang Y, et al.
**摘要**: 通过构建MT1F重组蛋白体外处理胃癌细胞，发现MT1F通过抑制TGF-β/Smad信号通路降低上皮-间质转化(EMT)标志物表达，进而抑制肿瘤细胞迁移和侵袭。研究为MT1F作为肿瘤治疗靶点提供了实验依据。
3. **文献名称**: "Structural Insights into the Metal-Binding Specificity of MT1F Isoform by NMR Spectroscopy"
**作者**: Gupta S, et al.
**摘要**: 利用核磁共振(NMR)技术解析了重组MT1F蛋白与锌、铜离子的结合构象，发现其C端结构域对铜离子具有独特亲和力。该研究揭示了MT1F与其他MT亚型(如MT1A/MT2A)在金属配位选择性和生理功能上的差异。
4. **文献名称**: "MT1F Recombinant Protein Attenuates Neuroinflammation in a Mouse Model of Parkinson’s Disease"
**作者**: Kim H, et al.
**摘要**: 在小鼠帕金森病模型中，腹腔注射重组MT1F蛋白显著降低了黑质区促炎因子(如TNF-α、IL-6)水平，并缓解了多巴胺能神经元损伤。研究表明MT1F可能通过调节NF-κB通路发挥神经保护作用。
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建议通过**PubMed**、**Web of Science**或**Google Scholar**检索关键词“MT1F recombinant protein”“Metallothionein 1F expression”等获取真实文献。
MT1F (Metallothionein 1F) is a member of the metallothionein (MT) protein family, which comprises small, cysteine-rich proteins that bind heavy metal ions through metal-thiolate clusters. These evolutionarily conserved proteins play critical roles in metal homeostasis, detoxification of toxic metals (e.g., cadmium, mercury), and protection against oxidative stress. The human MT family includes four major subfamilies (MT1–MT4), with MT1 further divided into multiple isoforms, including MT1F. MT1F shares the characteristic structure of MTs, containing 20 highly conserved cysteine residues within a 61–62 amino acid sequence, enabling strong metal-binding capacity for divalent cations like zinc (Zn²⁺) and copper (Cu²⁺).
Expression of MT1F is regulated by metal ions, glucocorticoids, and oxidative stress, primarily in tissues involved in metal metabolism, such as the liver, kidney, and intestines. Its functions extend beyond metal regulation, including scavenging reactive oxygen species (ROS), modulating immune responses, and influencing cell proliferation/apoptosis pathways. Dysregulation of MT1F has been implicated in pathologies such as cancer, neurodegenerative diseases, and metal toxicity. For example, MT1F overexpression has been observed in certain tumors, suggesting a potential role in chemoresistance or tumor microenvironment adaptation.
Recombinant MT1F proteins are engineered using expression systems like E. coli or yeast to study its biochemical properties and therapeutic potential. Production involves cloning the MT1F gene into expression vectors, optimizing induction conditions for metal-binding activity, and purifying the protein via chromatography. Recombinant MT1F serves as a tool for elucidating metal-related disease mechanisms, developing metal chelation therapies, or engineering metal-responsive biosensors. Ongoing research aims to harness its antioxidant and cytoprotective properties for biomedical applications.
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