| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FUCA1 |
| Uniprot No | P04066 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-466aa |
| 氨基酸序列 | QPPRRYTPD WPSLDSRPLP AWFDEAKFGV FIHWGVFSVP AWGSEWFWWH WQGEGRPQYQ RFMRDNYPPG FSYADFGPQF TARFFHPEEW ADLFQAAGAK YVVLTTKHHE GFTNWPSPVS WNWNSKDVGP HRDLVGELGT ALRKRNIRYG LYHSLLEWFH PLYLLDKKNG FKTQHFVSAK TMPELYDLVN SYKPDLIWSD GEWECPDTYW NSTNFLSWLY NDSPVKDEVV VNDRWGQNCS CHHGGYYNCE DKFKPQSLPD HKWEMCTSID KFSWGYRRDM ALSDVTEESE IISELVQTVS LGGNYLLNIG PTKDGLIVPI FQERLLAVGK WLSINGEAIY ASKPWRVQWE KNTTSVWYTS KGSAVYAIFL HWPENGVLNL ESPITTSTTK ITMLGIQGDL KWSTDPDKGL FISLPQLPPS AVPAEFAWTI KLTGVK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FUCA1重组蛋白的示例参考文献(均为虚构，仅供格式参考)：
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1. **文献名称**: "Recombinant Human α-L-Fucosidase (FUCA1): Expression in CHO Cells and Enzymatic Characterization"
**作者**: Smith A, et al.
**摘要**: 报道了在CHO细胞中重组表达人源FUCA1蛋白的方法，并验证其水解α-1.6-岩藻糖苷键的活性，为溶酶体贮积症研究提供工具。
2. **文献名称**: "Structural Analysis of Recombinant FUCA1 Reveals Substrate Specificity Determinants"
**作者**: Lee J, et al.
**摘要**: 通过X射线晶体学解析重组FUCA1的三维结构，揭示了其底物结合口袋的关键氨基酸残基，解释了突变导致岩藻糖苷贮积症的分子机制。
3. **文献名称**: "Therapeutic Potential of Recombinant FUCA1 in a Mouse Model of Fucosidosis"
**作者**: Brown K, et al.
**摘要**: 在小鼠模型中评估重组FUCA1的酶替代疗法效果，显示其能有效减少脑和肝脏中的岩藻糖苷积累，改善病理表型。
4. **文献名称**: "High-Yield Production of Active FUCA1 in E. coli Using Codon Optimization and Refolding Strategies"
**作者**: Zhang Y, et al.
**摘要**: 通过密码子优化和大肠杆菌表达系统实现重组FUCA1的高效可溶性表达，优化复性工艺后获得高活性酶，降低成本。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词：
`recombinant FUCA1 protein`、`α-L-fucosidase expression`、`fucosidosis therapy`。
**Background of Recombinant FUCA1 Protein**
FUCA1 (alpha-L-fucosidase 1) is a lysosomal enzyme encoded by the *FUCA1* gene in humans, located on chromosome 1p34. This enzyme catalyzes the hydrolysis of alpha-1.6-linked fucose residues from glycoproteins, glycolipids, and oligosaccharides, playing a critical role in cellular glycosphingolipid metabolism. Deficiencies in FUCA1 activity are linked to the rare autosomal recessive disorder fucosidosis, characterized by progressive neurodegeneration, developmental delays, and impaired lysosomal storage.
Recombinant FUCA1 protein is produced using biotechnological platforms (e.g., mammalian, bacterial, or insect cell systems) to express the purified enzyme for research and therapeutic applications. Its production typically involves cloning the *FUCA1* cDNA into expression vectors, followed by protein purification via affinity chromatography. Recombinant FUCA1 retains the enzymatic activity of the native protein, making it valuable for studying substrate specificity, enzyme kinetics, and mechanisms underlying fucosidosis.
In biomedical research, recombinant FUCA1 serves as a tool to investigate lysosomal storage disorders, develop enzyme replacement therapies (ERT), and screen potential pharmacological chaperones. It also aids in diagnosing fucosidosis by measuring enzyme activity in patient samples. Recent advances in protein engineering aim to optimize its stability, delivery, and efficacy for clinical use. Additionally, studies explore its role beyond lysosomal function, including potential implications in cancer metastasis and immune regulation, where fucose-containing glycans modulate cell-cell interactions.
Overall, recombinant FUCA1 bridges basic science and translational medicine, offering insights into glycosylation biology and therapeutic strategies for lysosomal disorders.
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