| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRA1 |
| Uniprot No | Q9UI14 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-185aa |
| 氨基酸序列 | MAAQKDQQKDAEAEGLSGTTLLPKLIPSGAGREWLERRRATIRPWSTFVDQQRFSRPRNLGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLESKLVLFGREVSPAHQYALAGGISFPFFWLAGAGSAVFWVLGATLVVIGSHAAFHQIEAVDGEELQMEPV |
| 预测分子量 | 20,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRA1重组蛋白的3篇代表性文献摘要概括(内容为模拟示例，仅供参考)：
1. **"Functional characterization of recombinant PRA1 in vesicular trafficking"**
- 作者：Smith et al.
- 摘要：研究通过在大肠杆菌中表达并纯化重组PRA1蛋白，验证其与Rab GTP酶(如Rab5和Rab7)的相互作用，证明PRA1在细胞内囊泡运输中的关键调控作用。
2. **"Structural insights into PRA1-mediated membrane tethering"**
- 作者：Zhang & Lee
- 摘要：利用重组PRA1蛋白进行晶体结构解析，揭示其N端结构域如何介导膜锚定，并阐明其参与高尔基体到内体运输的分子机制。
3. **"PRA1 knockdown and recombinant rescue in neuronal models"**
- 作者：Chen et al.
- 摘要：通过体外重组PRA1蛋白回补实验，证实其在神经元突触小泡循环中的必要性，为神经退行性疾病相关通路提供机制依据。
(注：以上文献为示例，实际文献需通过PubMed或Google Scholar等平台以关键词“PRA1 recombinant”“Yip1 protein trafficking”检索。)
PRA1 (Prenylation Factor Rab Acceptor 1), also known as Yip3 or PRAF2. is a conserved eukaryotic protein involved in intracellular membrane trafficking and vesicular transport. Initially identified in yeast, PRA1 homologs exist across species, including humans (where it is termed PRAF2 or YIPF3). This small, multifunctional protein (approximately 20 kDa) features four transmembrane domains and localizes to the Golgi apparatus, endoplasmic reticulum, and vesicular membranes. Its primary role centers on regulating Rab GTPases—key controllers of vesicle budding, motility, and fusion. PRA1 interacts with prenylated Rab proteins, influencing their membrane association, activation cycle, and spatial distribution.
Biochemically, PRA1 demonstrates dual functionality: it extracts GDP-bound Rab proteins from membranes and facilitates their solubilization through hydrophobic interactions, while also participating in Rab recycling via guanine nucleotide exchange factor (GEF)-like activity. This makes PRA1 critical for maintaining Rab homeostasis and ensuring proper vesicle trafficking pathways. Dysregulation of PRA1 has been implicated in neurological disorders and cancer progression, with altered expression observed in gliomas and hepatocellular carcinomas.
Recombinant PRA1 proteins are typically produced in E. coli or mammalian expression systems for structural and functional studies. Purified recombinant PRA1 serves as an essential tool for investigating Rab-mediated trafficking mechanisms, screening small molecule modulators, and developing diagnostic markers. Its conserved nature across eukaryotes makes it a valuable model for studying evolutionary adaptations in membrane trafficking systems. Current research focuses on resolving its 3D structure, mapping interaction interfaces with Rab isoforms, and exploring therapeutic targeting opportunities in trafficking-related diseases.
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