| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ly6c1 |
| Uniprot No | P0CW02 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-109aa |
| 氨基酸序列 | LQCYECYGVPIETSCPAVTCRASDGFCIAQNIELIEDSQRRKLKTRQCLS FCPAGVPIRDPNIRERTSCCSEDLCNAAVPTAG |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ly6c1重组蛋白的3篇参考文献的简要总结(注：文献为虚拟示例，实际引用请核实)：
1. **《Ly6c1重组蛋白的表达与功能研究》**
- 作者：Smith A, et al.
- 摘要：本研究通过原核表达系统成功制备了Ly6c1重组蛋白，并验证其在T细胞活化中的调控作用，发现其通过与特定膜受体结合抑制炎症信号通路。
2. **《Ly6c1的晶体结构解析及其配体结合特性》**
- 作者：Zhang L, et al.
- 摘要：通过X射线衍射解析了Ly6c1重组蛋白的三维结构，揭示了其独特的“三指”折叠模式，并鉴定出与胆固醇类分子结合的活性位点，为药物靶点开发提供依据。
3. **《Ly6c1在代谢性疾病模型中的功能缺失研究》**
- 作者：Chen R, et al.
- 摘要：利用重组Ly6c1蛋白干预高脂饮食小鼠模型，发现其能够改善胰岛素抵抗和脂肪肝症状，提示Ly6c1可能作为代谢性疾病的潜在治疗靶标。
如需具体文献，建议通过PubMed或Web of Science以“Ly6c1 recombinant protein”为关键词检索近年研究。
Ly6c1. a member of the lymphocyte antigen-6 (LY6)/urokinase-type plasminogen activator receptor (uPAR) protein superfamily, is a cell surface protein anchored by glycosylphosphatidylinositol (GPI). This family is characterized by conserved LU domains containing 8-10 cysteine residues that form disulfide bonds, enabling structural stability and interaction with ligands. Ly6c1 is primarily expressed in immune cells, including specific subsets of monocytes, T cells, and dendritic cells, though its expression patterns and biological roles remain less defined compared to other LY6 family members like Ly6C or Ly6G.
Recombinant Ly6c1 protein is engineered for in vitro studies to overcome challenges in isolating native proteins due to low natural abundance and membrane-associated localization. It is typically produced in heterologous expression systems (e.g., E. coli, mammalian cells) with tags (e.g., His-tag, Fc-fusion) to facilitate purification and detection. The recombinant form retains functional epitopes, enabling research into its interactions with ligands, receptors, or antibodies, as well as its role in immune modulation.
Functionally, Ly6c1 is implicated in cell adhesion, migration, and immune regulation. Studies suggest it may influence monocyte differentiation or T-cell activation, potentially through interactions with unknown partners. Its GPI anchor hints at localization to lipid rafts, which are critical for signal transduction. However, mechanistic insights are limited, partly due to overlapping roles with other LY6 proteins and a lack of specific inhibitors or antibodies.
Interest in Ly6c1 also stems from its association with diseases. For example, altered Ly6c1 expression has been observed in inflammatory conditions and cancer, though its exact contribution remains unclear. Recombinant Ly6c1 serves as a tool to dissect these pathways, aiding drug discovery or biomarker development. Further research is needed to clarify its physiological ligands, signaling cascades, and therapeutic potential.
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