| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAVER2 |
| Uniprot No | Q9HCJ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140aa |
| 氨基酸序列 | MAAAAGDGGGEGGAGLGSAAGLGPGPGLRGQGPSAEAHEGAPDPMPAALHPEEVAARLQRMQRELSNRRKILVKNLPQDSNCQEVHDLLKDYDLKYCYVDRNKRTAFVTLLNGEQAQNAIQMFHQYSFRGKDLIVQLQPT |
| 预测分子量 | 17.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAVER2重组蛋白的示例参考文献(基于已有知识推测，建议通过学术数据库核实最新研究)：
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1. **文献名称**: *RAVER2 interacts with PTB and regulates mRNA splicing in mammalian cells*
**作者**: Smith A, et al.
**摘要**: 本研究利用重组RAVER2蛋白进行体外结合实验，证实其通过PTB结合域与多嘧啶区结合蛋白(PTB)相互作用，参与调控pre-mRNA剪接过程。重组蛋白在HEK293细胞中过表达后，显著改变了特定靶基因的剪接模式。
2. **文献名称**: *Structural characterization of recombinant RAVER2 reveals RNA-binding motifs*
**作者**: Lee J, et al.
**摘要**: 通过在大肠杆菌系统中表达并纯化重组RAVER2蛋白，结合X射线晶体学解析其三维结构，揭示了其N端含有保守的RNA识别基序(RRM)，为解释其参与RNA代谢的分子机制提供结构基础。
3. **文献名称**: *RAVER2 knockdown impairs cell migration and is rescued by recombinant protein expression*
**作者**: Chen L, et al.
**摘要**: 研究显示，RAVER2缺失导致细胞迁移能力下降，而通过重组RAVER2蛋白回补可逆转表型。实验利用His标签重组蛋白进行功能恢复，表明RAVER2在细胞骨架调控中起关键作用。
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**备注**：以上文献为示例性质，实际研究请通过PubMed、Web of Science等平台以“RAVER2 recombinant protein”为关键词检索获取最新结果。
RAVER2 (ribonucleoprotein, PTB-binding 2) is a regulatory protein involved in RNA processing and splicing, primarily functioning as a co-repressor or adaptor molecule. It belongs to the RAVER protein family, characterized by their interaction with polypyrimidine tract-binding proteins (PTB), key regulators of alternative splicing. RAVER2 contains multiple RNA recognition motifs (RRMs) and a PTB-binding region, enabling it to bridge interactions between PTB and other ribonucleoprotein complexes. This protein plays a role in modulating pre-mRNA splicing decisions, particularly in cell differentiation, stress responses, and developmental processes.
The recombinant RAVER2 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains functional domains necessary for binding RNA and PTB. Researchers use it to investigate how RAVER2 fine-tunes splicing events, impacts gene expression networks, or contributes to diseases like cancer, where splicing dysregulation is common. Its recombinant form also aids in structural studies, interaction assays, and drug screening platforms targeting splicing machinery. Current research focuses on delineating its tissue-specific roles and potential therapeutic applications in splicing-related pathologies.
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