| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GADD45G |
| Uniprot No | O95257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159aa |
| 氨基酸序列 | MTLEEVRGQDTVPESTARMQGAGKALHELLLSAQRQGCLTAGVYESAKVLNVDPDNVTFCVLAAGEEDEGDIALQIHFTLIQAFCCENDIDIVRVGDVQRLAAIVGAGEEAGAPGDLHCILISNPNEDAWKDPALEKLSLFCEESRSVNDWVPSITLPE |
| 预测分子量 | 33.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GADD45G重组蛋白的示例参考文献(部分为模拟内容，供参考)：
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1. **"Recombinant GADD45G protein induces cell cycle arrest and enhances DNA repair in human keratinocytes"**
*Authors: Smith J, et al.*
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组GADD45G蛋白，发现其通过激活p38/JNK信号通路诱导细胞周期G2/M期阻滞，并促进紫外线诱导的DNA损伤修复，提示其在皮肤癌预防中的潜在作用。
2. **"Functional characterization of GADD45G as a tumor suppressor in hepatocellular carcinoma"**
*Authors: Zhang L, et al.*
**摘要**: 通过重组GADD45G蛋白处理肝癌细胞，证实其能显著抑制细胞增殖并诱导凋亡，机制涉及下调CDK1/cyclin B1复合物活性及抑制MAPK通路，为肝癌靶向治疗提供新思路。
3. **"GADD45G interacts with PCNA to modulate DNA damage response"**
*Authors: Lee S, et al.*
**摘要**: 利用重组GADD45G蛋白进行体外结合实验，发现其直接结合增殖细胞核抗原(PCNA)，增强DNA损伤后同源重组修复效率，揭示了GADD45G在基因组稳定性中的关键作用。
4. **"Recombinant GADD45G sensitizes leukemia cells to chemotherapy"**
*Authors: Wang Y, et al.*
**摘要**: 研究证明重组GADD45G蛋白与阿霉素联用可显著增强白血病细胞对化疗的敏感性，机制与调控Bcl-2/Bax凋亡通路及ROS生成相关，提示其作为化疗增敏剂的潜力。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等平台检索最新文献。如需真实文献，可检索关键词如"recombinant GADD45G protein"或"GADD45G overexpression"。
GADD45G (Growth Arrest and DNA Damage-Inducible 45 Gamma) is a stress-responsive protein belonging to the GADD45 family, which includes GADD45A, GADD45B, and GADD45G. These proteins play critical roles in cellular stress signaling, cell cycle regulation, DNA repair, apoptosis, and immune responses. GADD45G is encoded by the GADD45G gene, located on chromosome 9 in humans, and is rapidly induced by various stressors, including UV radiation, oxidative stress, hypoxia, and chemotherapeutic agents. Its expression is regulated by transcription factors such as p53. BRCA1. and NF-κB, linking it to tumor suppression and genomic stability.
Functionally, GADD45G interacts with key cell cycle regulators, including CDC2/cyclin B1. to induce G2/M arrest, allowing cells to repair DNA damage or initiate apoptosis if damage is irreparable. It also modulates MAPK signaling pathways and participates in demethylation processes by recruiting TET enzymes to specific genomic loci, influencing epigenetic regulation. Studies highlight its tumor-suppressive roles in cancers like hepatocellular carcinoma and glioblastoma, where its downregulation correlates with poor prognosis.
Recombinant GADD45G protein is engineered for research and therapeutic applications, typically produced in bacterial or mammalian expression systems to ensure proper folding and post-translational modifications. It serves as a tool to study DNA damage responses, cell cycle checkpoints, and epigenetic mechanisms. Additionally, its potential in cancer therapy is being explored, particularly in restoring GADD45G expression to counteract uncontrolled proliferation or sensitize resistant tumors to treatment. Quality-controlled recombinant variants are essential for reproducibility in mechanistic studies and drug development pipelines.
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