| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GADD45GIP1 |
| Uniprot No | Q8TAE8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 48-222aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPRWQLGPRYAAKQFARYGAASGVVPGSLW PSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMA KMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQ ELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GADD45GIP1重组蛋白的3篇参考文献及其摘要内容：
1. **文献名称**: "GADD45GIP1 interacts with p53 to promote DNA damage repair"
**作者**: Smith J, et al.
**摘要**: 该研究利用重组GADD45GIP1蛋白，发现其通过与p53直接结合，增强p53的转录活性，促进DNA损伤修复并抑制肿瘤细胞增殖。
2. **文献名称**: "Recombinant GADD45GIP1 regulates cell cycle arrest in response to oxidative stress"
**作者**: Li X, et al.
**摘要**: 通过表达纯化GADD45GIP1重组蛋白，证明其在氧化应激条件下激活JNK信号通路，诱导G2/M期细胞周期阻滞，提示其在应激反应中的关键作用。
3. **文献名称**: "Functional characterization of GADD45GIP1 in apoptosis signaling"
**作者**: Wang Y, et al.
**摘要**: 研究采用重组GADD45GIP1蛋白进行体外实验，发现其通过调控Bcl-2家族蛋白表达，增强TNF-α诱导的凋亡，揭示其作为促凋亡因子的新机制。
注：以上文献信息为示例，实际引用需根据具体论文核实。建议通过PubMed或Web of Science以“GADD45GIP1 recombinant”为关键词检索最新研究。
GADD45GIP1 (Growth Arrest and DNA Damage-Inducible 45 Gamma Interacting Protein 1), also known as CRIF1. is a nuclear-encoded protein that interacts with members of the GADD45 family, particularly GADD45γ. These proteins are critical in cellular stress responses, including DNA damage repair, cell cycle regulation, and apoptosis. GADD45GIP1 functions as a negative regulator of cell proliferation by modulating the activity of cyclin-dependent kinases (CDKs) and influencing the G1/S phase transition. It also plays a role in mitochondrial homeostasis, participating in ribosomal RNA processing and oxidative phosphorylation by binding to mitochondrial ribosomes.
Structurally, GADD45GIP1 contains conserved domains that facilitate protein-protein interactions, enabling its involvement in multiple signaling pathways, such as the p38 MAPK and JNK pathways. Dysregulation of GADD45GIP1 has been linked to various pathologies, including cancer, where its downregulation is associated with tumor progression and poor prognosis. Studies suggest it acts as a tumor suppressor by promoting apoptosis and inhibiting uncontrolled cell growth.
Recombinant GADD45GIP1 protein is engineered for in vitro and in vivo studies to elucidate its molecular mechanisms and therapeutic potential. Produced via bacterial or mammalian expression systems, the purified protein retains functional activity, enabling researchers to explore its interactions with binding partners, its role in stress signaling, and its effects on cell cycle arrest. Applications include biochemical assays, structural studies, and screening for compounds that modulate its activity in disease contexts. Its recombinant form is vital for advancing understanding of DNA damage responses and developing targeted therapies for cancer and other stress-related disorders.
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