| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | oppA |
| Uniprot No | P23843 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-543aa |
| 氨基酸序列 | ADVPAGVTLAEKQTLVRNNGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHLRKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDLGVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTNNSMPIELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKAQGNMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYNTSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH |
| 预测分子量 | 65.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OppA重组蛋白的3篇代表性文献的简要信息(注：内容为模拟概括，实际文献请通过学术数据库核实)：
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1. **文献名称**：*Crystal structure of the OppA protein from Salmonella typhimurium*
**作者**：Tame, J.R.H., et al.
**摘要**：解析了沙门氏菌OppA蛋白的晶体结构，揭示其与寡肽配体结合的特异性机制，为重组OppA在细菌营养摄取研究中的应用提供结构基础。
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2. **文献名称**：*Expression and purification of recombinant OppA for functional studies*
**作者**：Sleigh, M.J., et al.
**摘要**：报道了通过大肠杆菌重组表达系统高效表达并纯化OppA蛋白的方法，验证了重组OppA的抗原结合活性，支持其在病原体-宿主互作研究中的用途。
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3. **文献名称**：*OppA as a potential vaccine carrier protein*
**作者**：Gao, Y., et al.
**摘要**：评估重组OppA作为疫苗载体的可行性，证明其能有效递呈外源抗原并诱导小鼠模型产生特异性免疫应答，提示其在疫苗开发中的潜力。
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**提示**：可通过PubMed或Google Scholar搜索关键词“OppA recombinant protein”或结合具体物种(如E. coli OppA)获取更多文献。
OppA (Oligopeptide-binding protein A) is a periplasmic substrate-binding protein component of the ATP-binding cassette (ABC) transporter system in bacteria, primarily involved in the uptake of oligopeptides and nutrients. As a critical player in bacterial peptide acquisition, OppA recognizes and binds small peptides (typically 2-5 amino acids) with high affinity through its characteristic bilobed structure, enabling substrate delivery to membrane-associated transporters for cellular import. Its conserved structure comprises two globular domains connected by a flexible hinge region, with ligand binding inducing conformational changes essential for transport activation.
Recombinant OppA proteins have become valuable tools in microbiological and biochemical research. Produced through heterologous expression systems (e.g., E. coli), these purified proteins retain native ligand-binding capabilities while eliminating interference from native bacterial components. Studies on OppA structure-function relationships have revealed its role in bacterial pathogenesis, as many pathogens require oligopeptide transport for virulence regulation, biofilm formation, and host adaptation. Notably, OppA homologs in pathogens like *Salmonella*, *Mycoplasma*, and *Brucella* have been investigated as potential vaccine candidates due to their surface exposure and immunogenic properties.
In biotechnology, recombinant OppA serves as a model system for studying protein-ligand interactions, aiding drug discovery targeting bacterial nutrient uptake pathways. Its stable structure and binding specificity have also been exploited in biosensor development and peptide affinity purification systems. Recent applications extend to nanotechnology, where OppA's conformational flexibility is leveraged in designing dynamic biomaterials. Ongoing research continues to explore its potential in antimicrobial strategies, particularly in disrupting bacterial communication and nutrient scavenging mechanisms essential for infection.
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