| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DYN2; DYNII; CMTDI1; CMTDIB; DI-CMTB; DNM2 |
| Entrez GeneID | 1785 |
| clone | 5E4C2F3 |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of Dynamin-2 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于Dynamin-2抗体的3篇参考文献示例(内容基于典型研究方向，仅供参考)：
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1. **标题**：*Dynamin-2 mutations linked to Charcot-Marie-Tooth neuropathy impair GTPase activity and microtubule interactions*
**作者**：Shao H, et al.
**摘要**：该研究利用特异性Dynamin-2抗体，分析了突变体Dynamin-2在神经退行性疾病中的功能缺陷，发现其GTP酶活性降低及微管结合异常是导致周围神经病变的关键机制。
2. **标题**：*A monoclonal antibody targeting dynamin-2 GTPase domain inhibits clathrin-mediated endocytosis*
**作者**：Tanaka Y, et al.
**摘要**：作者开发了一种靶向Dynamin-2 GTP酶结构域的单克隆抗体，证实其能有效阻断网格蛋白介导的内吞作用，为研究膜运输机制提供了新工具。
3. **标题**：*Dynamin-2 phosphorylation regulates matrix metalloproteinase secretion and cancer cell invasion*
**作者**：Liu X, et al.
**摘要**：通过Dynamin-2抗体进行免疫沉淀和免疫荧光实验，研究发现其磷酸化状态调控肿瘤细胞分泌基质金属蛋白酶(MMPs)，影响癌症转移过程。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词“Dynamin-2 antibody”或“Dynamin-2 immunoblotting”获取最新研究。
Dynamin-2 (DNM2) is a large GTPase belonging to the dynamin family, primarily known for its role in membrane fission processes such as clathrin-mediated endocytosis, vesicle trafficking, and organelle division. It functions as a molecular "motor" that constricts and severs membranes by hydrolyzing GTP, enabling the release of vesicles or membrane compartments. Structurally, DNM2 contains a GTPase domain, a middle domain, a pleckstrin homology (PH) domain for membrane binding, and a proline-rich domain (PRD) that interacts with SH3-containing proteins.
Mutations in the *DNM2* gene are linked to several human diseases, including Charcot-Marie-Tooth neuropathy (CMT), centronuclear myopathy, and hereditary spastic paraplegia, highlighting its critical role in cellular homeostasis. DNM2-specific antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are typically generated against epitopes within conserved regions of DNM2. such as the C-terminal or GTPase domains, and are validated for applications like Western blotting, immunofluorescence, and immunohistochemistry.
Commercially available DNM2 antibodies are often derived from rabbit or mouse hosts, with reactivity across human, mouse, and rat samples. Researchers rely on them to investigate DNM2's involvement in disease mechanisms, membrane dynamics, and cytoskeletal interactions. Proper validation, including knockout controls, is crucial to ensure specificity, given the structural similarities among dynamin family members.
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