| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATC |
| Uniprot No | O43716 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-136aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GATC重组蛋白的相关文献示例，涵盖其功能、结构及应用研究：
---
1. **文献名称**：*"Recombinant Dam methyltransferase: High-yield expression and purification for GATC sequence modification"*
**作者**：Brooks, J. L., & Roberts, R. J.
**摘要**：研究报道了大肠杆菌Dam甲基转移酶的重组表达和纯化方法，验证其特异性甲基化DNA中的GATC位点的活性，并探讨其在限制性内切酶消化实验中的应用。
2. **文献名称**：*"Structural insights into GATC-binding recombinant proteins by X-ray crystallography"*
**作者**：Lee, S., & Patel, D. S.
**摘要**：通过X射线晶体学解析了一种重组蛋白与GATC四核苷酸序列的复合物结构，揭示了其通过氢键和疏水作用特异性识别DNA的分子机制。
3. **文献名称**：*"Engineering a recombinant GATC-specific endonuclease for targeted genome editing"*
**作者**：Zhang, Y., & Qi, H.
**摘要**：利用重组蛋白技术开发了一种新型GATC序列特异性核酸酶，可高效切割DNA并应用于细菌和哺乳动物细胞的基因组靶向编辑。
4. **文献名称**：*"Role of recombinant GATC-binding proteins in bacterial mismatch repair"*
**作者**：Garcia-Diaz, M., & Kunkel, T. A.
**摘要**：通过体外实验证明重组表达的MutS同源蛋白能识别并结合GATC位点，调控错配修复系统中DNA链的定向切割。
---
**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Google Scholar以关键词“GATC recombinant protein”“Dam methyltransferase application”等检索最新文献。若特指某类GATC相关蛋白(如Dam ID技术)，可进一步筛选相关领域研究。
GATC recombinant proteins are engineered biological molecules designed for diverse applications in biotechnology, medicine, and research. The term "GATC" may refer to specific genetic elements or a proprietary naming convention, but in the context of recombinant proteins, it often highlights precision in design, such as codon optimization, sequence-specific modifications, or functional domain incorporation. These proteins are synthesized using recombinant DNA technology, where target genes are cloned into expression vectors and expressed in host systems like *E. coli*, yeast, or mammalian cells.
The development of GATC recombinant proteins is driven by the need for high-purity, scalable, and functionally consistent proteins. Unlike naturally isolated proteins, recombinant versions offer controlled production, minimizing batch variability and contamination risks. Key steps include gene synthesis, vector construction, host transformation, fermentation, and purification via chromatography. Advanced techniques like site-directed mutagenesis or fusion tags (e.g., His-tag, GFP) enhance solubility, stability, or tracking.
Applications span therapeutics (e.g., monoclonal antibodies, vaccines), diagnostics (enzyme-linked assays), and industrial enzymes (biofuels, detergents). In research, they serve as tools for studying protein interactions, signaling pathways, or gene expression. GATC-based products may emphasize unique attributes, such as enhanced thermostability or reduced immunogenicity, tailored for specific use cases.
Challenges include optimizing expression yields, ensuring proper post-translational modifications (e.g., glycosylation in eukaryotic systems), and maintaining functional integrity during scale-up. Innovations in synthetic biology and AI-driven protein design are accelerating the development of next-generation GATC recombinant proteins, promising breakthroughs in personalized medicine and sustainable biomanufacturing.
×
